Paper Information

Journal:   IRANIAN JOURNAL OF PUBLIC HEALTH   AUGUST 2014 , Volume 43 , Number SUPPLEMENT 2; Page(s) 74 To 74.
 
Paper: 

PLASMID-MEDIATED QUINOLONE RESISTANCE DETERMINANTS QNR AND AAC (6?) - IB-CR IN FLUOROQUINOLONE-RESISTANT ENTEROBACTERIACEAE IN HAMADAN, IRAN

 
 
Author(s):  KAMALI KAKHKI REZA*, ALIKHANI MOHAMMAD YOUSEF, YOUSEFI MASHOUF RASOUL, ROOINTAN AMIR, ABAZARI MALEK
 
* MICROBIOLOGY DEPARTMENT, HAMEDAN UNIVERSITY OF MEDICAL SCIENCES, HAMEDAN, IRAN
 
Abstract: 

Background: The quinolone group is an important class of broad-spectrum antimicrobial agents. Plasmid mediated quinolone resistance (PMQR) determinants have emerged as a significant concern in recent years. This study reports the screening of resistant-isolates to Fluoroquinolone antimicrobial agents for PMQR determinants and detection of Qnr and aac (6?) -Ib-cr genes.
Methods: Total of 100 fluoroquinolone-resistant Enterobacteriaceae were isolated from 3 hospitals in Hamadan from October 2012 to June 2013. The isolates were identified by biochemical tests and confirmed by PCR. Antimicrobial susceptibility to 14 antimicrobial agents including levofloxacin and ciprofloxacin was determined by Clinical Laboratory Standards Institute (CLSI). Disk diffusion methods and ciprofloxacin MIC were obtained by broth microdilution. Then the isolates were screened for the presence of qnrA, qnrB, qnrS and aac (6?) -Ib-cr genes using PCR assay. Results: Among the screened isolates, 64 strains (64%) of Escherichia coli, 23 strains (23%) of Klebsiella pneumoniae, 13 strains (13%) of Proteus mirabilis were collected as quinolone- resistant isolates. Out of 100 isolates, two (2%) isolates were positive for qnrS, seventeen (17%) isolates were positive for qnrB and we did not find the qnrA gene in any of the isolates. There were also 32 positive isolates for aac (6') -Ib-cr gene.
Conclusion: In this study we describe the prevalence of qnr and aac (6?) -Ib-cr genes in Fluoroquinolone-resistant Enterobacteriaceae in Hamadan. The carriage rate of multidrug resistant Enterobacteriaceae in healthy people in Hamadan City is extremely high. Moreover, genes encoding transferable quinolones, in particular aac (6?) -Ib-cr, are highly prevalent in these strains.

 
Keyword(s): ENTEROBACTERIACEAE, ANTIBIOTIC RESISTANCE, FLUOR QUINOLONE
 
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