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Paper Information

Journal:   IRANIAN JOURNAL OF PUBLIC HEALTH   AUGUST 2014 , Volume 43 , Number SUPPLEMENT 2; Page(s) 74 To 74.
 
Paper: 

MOLECULAR STUDY OF ERYTHROMYCIN RESISTANCE FACTORS IN GRAM POSITIVE COCCI ISOLATED FROM IN SHAHID RAJAEE TEACHING HOSPITAL, QAZVIN

 
 
Author(s):  MOOSAVI MIRESMAEL*, PEYMANI AMIR, MOOSAVI RAZIYEH
 
* DEPARTMENT OF MEDICAL MICROBIOLOGY, QOM UNIVERSITY OF MEDICAL SCIENCES, QOM, IRAN
 
Abstract: 

Background: Gram positive cocci organisms show many molecular important resistance factors to erythromycin which would be important nosocomial infections agents. This study was conducted to determine the molecular factors for resistance to erythromycin, in gram positive cocci isolated from laryngoscope in Shahid Rajaee teaching hospital, Qazvin.
Methods: This molecular descriptive study was carried on the samples collected from the laryngoscope in 1392. In aseptic conditions; samples were transferred to microbiology laboratory. Bacterial identification was performed usingthe standard laboratory methods. Antibiotic sensitivity was carried out using usual disk diffusion agar method according to CLSI recommendation. PCR assay was performed and and the products of it was sequenced to check the presence of mef, msrA, erm A, erm B, ermC genes.
Results: 21 isolates (41/1%) out of 51 were resistance to erythromycin. In Coagulase –ve Staphylococci (CONS) were positive for ermC (4-19.5%) and mef (1-4.76%), respectivily. Alpha hemolytic Streptococci were also positive for both ermA and ermB (2-9.52%) and gamma hemolytic Streptococci carried ermB (4-19.5%), ermA and mef (1-4.76%).
Conclusion: The most important finding in this study is the presence of the erythromycin resistance genes for the mef, msrA, ermA, ermB, erm C in collected isolates from laryngoscope in our hospital.Since laryngoscope can potentially be carried in resistant isolates, disinfection and control the infection is essential before using this device in patients.

 
Keyword(s): LARYNGOSCOPE, GRAM POSITIVE COCCI, ERYTHROMYCIN, RESISTANT GENES
 
 
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APA: Copy

MOOSAVI, M., & PEYMANI, A., & MOOSAVI, R. (2014). MOLECULAR STUDY OF ERYTHROMYCIN RESISTANCE FACTORS IN GRAM POSITIVE COCCI ISOLATED FROM IN SHAHID RAJAEE TEACHING HOSPITAL, QAZVIN. IRANIAN JOURNAL OF PUBLIC HEALTH, 43(SUPPLEMENT 2), 74-74. https://www.sid.ir/en/journal/ViewPaper.aspx?id=410765



Vancouver: Copy

MOOSAVI MIRESMAEL, PEYMANI AMIR, MOOSAVI RAZIYEH. MOLECULAR STUDY OF ERYTHROMYCIN RESISTANCE FACTORS IN GRAM POSITIVE COCCI ISOLATED FROM IN SHAHID RAJAEE TEACHING HOSPITAL, QAZVIN. IRANIAN JOURNAL OF PUBLIC HEALTH. 2014 [cited 2021November27];43(SUPPLEMENT 2):74-74. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=410765



IEEE: Copy

MOOSAVI, M., PEYMANI, A., MOOSAVI, R., 2014. MOLECULAR STUDY OF ERYTHROMYCIN RESISTANCE FACTORS IN GRAM POSITIVE COCCI ISOLATED FROM IN SHAHID RAJAEE TEACHING HOSPITAL, QAZVIN. IRANIAN JOURNAL OF PUBLIC HEALTH, [online] 43(SUPPLEMENT 2), pp.74-74. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=410765.



 
 
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