Paper Information

Journal:   IRANIAN JOURNAL OF PUBLIC HEALTH   AUGUST 2014 , Volume 43 , Number SUPPLEMENT 2; Page(s) 73 To 73.
 
Paper: 

RAPID DETECTION OF EXTENDED SPECTRUM ?-LACTAMASES (ESBLS) PRODUCING ISOLATES OF KLEBSIELLA PNEUMONIA BY A NEW COLORIMETRIC MEDIUM

 
 
Author(s):  SHAHSAVARI SHIRIN*, SALMANZADEH AHRABI SIAVOSH, FALSAFI TAHEREH, IRAJIAN GHOLAM REZA
 
* DEPARTMENT OF MICROBIOLOGY, FACULTY OF SCIENCES, ALZAHRA UNIVERSITY, TEHRAN, IRAN
 
Abstract: 

Background: The purpose of this study was to evaluate a new rapid colorimetric method for detection of ESBL producing Klebsiella pneumoniae. This method is capable to detect ESBL producing bacteria, by a color change in the colorimetric medium within 5 to 6 hours, which is due to metabolic activity of growing bacteria. The method is based on CLSI standard disk diffusion method performed on the colorimetric medium.
Methods: Fifty-four clinical isolates of Klebsiella pneumoniae obtained from Pasteur Institute of Iran and Iran University of Medical science were used for evaluation of this medium. 25 ESBL positive and 29 ESBL isolates were compared by CLSI disk diffusion ESBL (phenotypic confirmatory test) criteria by Mueller Hinton and colorimetric medium. The tested antibiotics included ceftazidim (CAZ), ceftazidime + clavulanate (CZA), cefotaxime (CTX), cefotaxime +clavulanate (CTC).
Results: A color change was observed for ESBL producing bacteria within 5 to 6 hours by the colorimetric medium. These results were in line with the results of overnight incubation on Mueller Hinton agar.
Conclusion: This new colorimetric medium can be used for rapid and reliable detection of ESBL producing Klebsiella pneumoniae within 5 to 6 hours.

 
Keyword(s): COLORIMETRIC MEDIUM, RAPID DETECTIONS, CLSI DISK DIFFUSION TEST
 
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