Click for new scientific resources and news about Corona[COVID-19]

Paper Information

Journal:   IRANIAN JOURNAL OF PUBLIC HEALTH   AUGUST 2014 , Volume 43 , Number SUPPLEMENT 2; Page(s) 72 To 72.
 
Paper: 

DEVELOPMENT OF ENZYME-LINKED IMMUNOSORBENT ASSAY WITH A NUCLEOPROTEIN AS AN ANTIGEN FOR DETECTION OF ANTIBODIES AGAINST AVIAN INFLUENZA VIRUS

 
 
Author(s):  JAYDARI AMIN*, SEYFI ABAD SHAPOURI M.R.
 
* DEPARTMENT OF MICROBIOLOGY,FACULTY OF VETERINARY MEDICINE, LORESTAN UNIVERSITY, KHORRAMABAD, IRAN
 
Abstract: 

Background: The aim of this study was to produce recombinant nucleoprotein (NP) and development of an ELISA test with it.
Methods: For this purpose, the coding region of nucleoprotein (NP) gene of A/chicken/Iran/AH-1/06 (H9N2) AI isolate was amplified by RT-PCR and cloned into a prokaryotic expression vector (pMal-C2x) and transformed into E. coli BL21 (DE3). The recombinant protein MBP-NP was expressed in E. coli and was purified using amylase resin chromatography column and used as the diagnostic antigen to develop a NP-based type specific indirect enzyme linked immunosorbent assay (ELISA) for detecting antibodies to AI for chicken sera. The NP-ELISA was compared with a commercial AIV ELISA kit (IDEXX- USA).
Results: Excellent agreement was obtained between NPELISA and commercial ELISA kit (k= 0.932). Results indicated that in house NP- ELISA is more sensitive than commercial ELISA for detecting the antibodies in chicken inflected with AIV but the difference was not significant.
Conclusion: This finding indicates that the indirect ELISA based on rNP developed in our laboratory is a sensitive and specific test and can be used for diagnosis and seroepidemiological investigation in long-term AIV program prevention and control.

 
Keyword(s): AVIAN INFLUENZA VIRUS, NUCLEOPROTEIN, H9N2, NP-ELISA
 
References: 
  • ندارد
 
  Yearly Visit 40
 
Latest on Blog
Enter SID Blog