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Journal:   IRANIAN JOURNAL OF PUBLIC HEALTH   AUGUST 2014 , Volume 43 , Number SUPPLEMENT 2; Page(s) 71 To 71.
 
Paper: 

Development And Clinical Validation Of Loop-Mediated Isothermal Amplification Method For Rapid Detection Of Bordetella Pertussis Based On Insertion Sequence Is481

 
 
Author(s):  Allahyar Torkaman Mohammad Reza*, Nikbin Vajihe Sadat, Nakhost Lotfi Masoumeh, Shahcheraghi Fereshteh
 
* DEPARTMENT OF MICROBIOLOGY, MIC RESEARCH CENTER, PASTEUR INSTITUTE OF IRAN, TEHRAN, IRAN
 
Abstract: 
Background: In spite of the existence of conventional culture method and developing PCR-based assays, yet the accurate detection of Bordetella pertussis is a problem. In this study, we developed a Loop-Mediated Isothermal Amplification (LAMP) assay based on IS481 and it has evaluated the efficiency, rapidity, sensitivity, and specificity of IS481 LAMP in comparison with real-time PCR with the same target for the detection of B. pertussis clinical specimens.Methods: One hundred and sixty-five nasopharyngeal swabs were used to be assessed by LAMP and real-time PCR. Amplified LAMP products were detected by adding SYBRR Safe DNA Gel Stain. Illumination under UV light and the green color for positive samples were visible by unarmed eyes.Results: The sensitivity and specificity of the LAMP assay were reasonable in comparison with real-time PCR. In this study, they were 87.5% and 92.9% for IS481 target, respectively. Amplification of 10 fg by IS481-LAMP showed the sensitivity of 2.26 genomic copies of B. pertussis.Conclusion: The developed LAMP assay based on IS481 target helps rapid and sensitive detection of B. pertussis, and it is cheaper than previous conventional real-time PCR. This IS-481 LAMP assay has the merit of being used as a diagnostic method for B. pertussis.
 
Keyword(s): LAMP, BORDETELLA PERTUSSIS, IS481
 
 
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APA: Copy

ALLAHYAR TORKAMAN, M., & NIKBIN, V., & NAKHOST LOTFI, M., & SHAHCHERAGHI, F. (2014). DEVELOPMENT AND CLINICAL VALIDATION OF LOOP-MEDIATED ISOTHERMAL AMPLIFICATION METHOD FOR RAPID DETECTION OF BORDETELLA PERTUSSIS BASED ON INSERTION SEQUENCE IS481. IRANIAN JOURNAL OF PUBLIC HEALTH, 43(SUPPLEMENT 2), 71-71. https://www.sid.ir/en/journal/ViewPaper.aspx?id=410754



Vancouver: Copy

ALLAHYAR TORKAMAN MOHAMMAD REZA, NIKBIN VAJIHE SADAT, NAKHOST LOTFI MASOUMEH, SHAHCHERAGHI FERESHTEH. DEVELOPMENT AND CLINICAL VALIDATION OF LOOP-MEDIATED ISOTHERMAL AMPLIFICATION METHOD FOR RAPID DETECTION OF BORDETELLA PERTUSSIS BASED ON INSERTION SEQUENCE IS481. IRANIAN JOURNAL OF PUBLIC HEALTH. 2014 [cited 2022August14];43(SUPPLEMENT 2):71-71. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=410754



IEEE: Copy

ALLAHYAR TORKAMAN, M., NIKBIN, V., NAKHOST LOTFI, M., SHAHCHERAGHI, F., 2014. DEVELOPMENT AND CLINICAL VALIDATION OF LOOP-MEDIATED ISOTHERMAL AMPLIFICATION METHOD FOR RAPID DETECTION OF BORDETELLA PERTUSSIS BASED ON INSERTION SEQUENCE IS481. IRANIAN JOURNAL OF PUBLIC HEALTH, [online] 43(SUPPLEMENT 2), pp.71-71. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=410754.



 
 
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