Paper Information

Journal:   IRANIAN JOURNAL OF PUBLIC HEALTH   AUGUST 2014 , Volume 43 , Number SUPPLEMENT 2; Page(s) 70 To 70.
 
Paper: 

COMPARISON OF FOUR DIAGNOSTIC METHODS FOR DETECTION OF METHICILLINRESISTANT STAPHYLOCOCCUS AUREUS

 
 
Author(s):  HASANZADEH SEPIDEH*, POURMAND MOHAMMAD REZA, MASHHADE RAHIL, DEHBASHI SANAZ
 
* BIOTECHNOLOGY RESEARCH CENTER, TEHRAN UNIVERSITY OF MEDICAL SCIENCES, TEHRAN, IRAN
 
Abstract: 

Background: Methicillin-resistant Staphylococcus aureus (MRSA) is a well-known pathogen with a worldwide distribution. Due to the increasing rate of MRSA infections, implementing of reliable, accurate and rapid testing for diagnosis of MRSA is necessary. The aim of this study was to compare four diagnostic methods for detection of MRSA isolates.
Methods: From December 2012 to April 2014, we collected 120 S. aureus isolates from three hospitals affiliated with Tehran University of Medical Sciences. MRSA isolates were detected by four different methods including cefoxitin disc diffusion test, oxacillin disc diffusion test, minimum inhibitory concentration (MIC) of oxacillin as determined by MIC test strip, and mecA detection by PCR. Results: Out of 120 S. aureus isolates, cefoxitin disc diffusion test, oxacillin disc diffusion test and MIC test strip identified 60 (50%), 48 (40%), 55 (45.83%) isolates as MRSA, respectively. The sensitivity and specificity for oxacillin disc diffusion, cefoxitin disc diffusion and MIC of oxacillin were 80% and 100%, 100% and 100%, and 91.6% and 100%, respectively.
Conclusion: Cefoxitin disc diffusion test is a reliable substitute for detection of MRSA in clinical laboratory where MIC detection and molecular methods are not accessible.

 
Keyword(s): STAPHYLOCOCCUS AUREUS, METHICILLIN RESISTANCE, MICROBIAL SENSITIVITY TESTS
 
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