Background: Salmonellosis is one of the most common food-induced diseases which is widely distributed all over the world and is known as one of the most serious public health concerns. Therefore, it is necessary to obtain developed methods in order to achieve a quick response in identifying Salmonella bacterium. This study aimed to evaluate hisD genes as a potential tool for early recognition of Salmonella enterica serotype Infantis.
Methods: Examined isolates in this study were separated from patients with Salmonella enterica serotype Infantis infection in several hospitals of Tehran and were prepared for polymerase chain reaction following bacteriological and biological tests. Afterward, a specific pair of primer was designed for hisD gene amplification by means of related software. Following isolates` genome extraction, PCR was done in order to identify Salmonella enterica serotype Infantis.
Results: Following the experiments above, the results of PCR product electrophoresis revealed a 651bp bond for hisD gene and primers didn't make a bond with enterobacteriacea strains such as Shigella and E.coli.
Conclusion: According to the results, hisD gene has the ability to recognize and identify Salmonella enterica serotype Infantis strains and it is also able to differentiate it from the rest of enterobacteriacea strains.