Paper Information

Journal:   IRANIAN JOURNAL OF PUBLIC HEALTH   AUGUST 2014 , Volume 43 , Number SUPPLEMENT 2; Page(s) 68 To 68.
 
Paper: 

ELECTROCHEMICAL AND BIOLOGICAL TEMPLATE: RAPID TECHNIQUE FOR DETECTION OF ECOLI TG

 
 
Author(s):  ESKANDARI KHADIJEH*, RAMAZANI MANIZHEH, FASIHI MEHDI, KAMALI MEHDI, DASHTESTANI FARIBA
 
* NANOBIOTECHNOLOGY RESEARCH CENTER, BAQIYATALLAH UNIVERSITY OF MEDICAL SCIENCE, TEHRAN, IRAN
 
Abstract: 

Background: The development of rapid and effective tools for the identification and quantitation of E. coli is of extreme importance in food analysis, environmental monitoring and clinical diagnostics.
Methods: The biosensor developed in this work is based on electrochemistry reactions. Therefore, the layout of the sensor is patterned as a two-electrode configuration, working electrode (WE) and reference electrode (RE). The RE were prepared by mixing 1.20 g of graphite powder, which had been heated at 700oC in a muffle furnace for 15 s, with 800
mL of paraffin oil with a mortar and pestle. A WE was prepared in a similar fashion, except that the graphite powder was mixed with a desired weight of bacteriophage. Both RE and WE pastes were packed into a polyethylene tube (2.5 mm diameter), the tip of which had been cut off. Electrical contact to the paste was established via inserting a copper wire thorough flank.
Results: Electrochemical experiments were carried out with an electrochemical interface LCR meter as a signal transducer and an electrochemical cell that contains the two-electrode system. The E. coli trapping on bacteriophage was reported by capacitance measurements. Conclusion: In this work, we have successfully fabricated an electrochemical biosensor with bacteriophage electrodes on a paste substrate. The proposed sensors have good characteristics such as; low detection limit, wide concentration, fast response time and good selectivity coefficient for Escherichia coli detection.

 
Keyword(s): BACTERIOPHAGE, E. COLI, LCR METER
 
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