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Paper Information

Journal:   IRANIAN JOURNAL OF PUBLIC HEALTH   AUGUST 2014 , Volume 43 , Number SUPPLEMENT 2; Page(s) 67 To 67.
 
Paper: 

MOLECULAR STUDY OF BRUCELLOSIS IN ENDEMIC REGIONS OF IRAN BASED ON NOVEL URS-PCR METHOD

 
 
Author(s):  ALAMIAN SAEED, ESMAELIZAD MAJID, AETEMADY AFSHAR
 
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Abstract: 

Background: Brucellosis is an infectious disease in human and animals. Bacteriologic-culture-based methods used for Brucella diagnosis are hard and time consuming. The aim of this study was to introduce a novel molecular method for detection of two prevalent species of Brucella in Iran, Brucella abortus and Brucella melitensis.
Methods: All complete sequences of chromosome 1 with 2.1 Mbp length were compared with all available Brucella sequences. A unique loci was found in chromosome one which was capable to differentiate Brucella abortus from Brucella melitansis. A set of primers was designed in flanking of unique loci. A total number of 136 (88 human and 48 bovine) well-characterised Brucella strains have been evaluated and classified by PCR method.
Results: Results of biochemical tests and bacteriophage typing as golden standard indicated that all Brucella strains isolated from human and bovine were B. melitensis biovar 1 and B. abortus biovar 3 respectively. The PCR results were the same as bacterilogical method in detection of the Brucella species.
Conclusion: Sensitivity and specificity assay indicated this method is suitable for detection of Brucella abortus and Brucella melitensis. Note that bacteriological methods are time consuming, need special equipment and conditions for detection of Brucella strains, thus we suggest that this novel PCR method, which was designed, based on the specific loci in chromosome 1 could be used for a rapid detection of Brucella melitensis and B.melitensis. The advantage of this method over other presented methods is that both B. abortus and B.melitensis is detectable using one-set primer pair simultaneously.

 
Keyword(s): BRUCELLA ABORTUS, BRUCELLA MELITENSIS, DETECTION, NOVEL PCR METHOD
 
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