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Paper Information

Journal:   IRANIAN JOURNAL OF PUBLIC HEALTH   AUGUST 2014 , Volume 43 , Number SUPPLEMENT 2; Page(s) 66 To 66.
 
Paper: 

ENTEROTOXAEMIA CAUSED BY CLOSTRIDIUM PERFRINGENS TYPE A IN GAZELLE

 
 
Author(s):  SHAMSADDINI BAFTI MEHRDAD, EZZATKHAH M., RASHIDI H., AMINI M.*, MOLLAEI M., TALEBHEMMAT M.
 
* RAZI VACCINE AND SERUM RESEARCH, KERMAN BRANCH, KERMAN, IRAN
 
Abstract: 

Background: Clostridium perfringensis a gram-positive anaerobic bacterium that is divided into five subtypes. All subtypes produce alpha toxin. In addition, type C produces beta toxin and type D produces epsilon toxin. Type A strains producing only alpha toxin is widespread in the intestines of warmblooded animals and in the environment. Strains of type A are associated with a wide variety of disease processes in many organ systems of myriad species of domestic animals. The present study describes a natural case of type A enterotoxemia in one gazelle in Kerman.
Methods: A dead, 1-year-old male gazelle was submitted to Razi Research Institute and examined. In necropsy, there was a severe hemorrhage in the serosa of abomasum and small intestine. For bacteriological analysis, a loopful of ingesta from thesmall intestine was immediately inoculated onto blood agar and incubated anaerobically, at 37oC. After 48 h of incubation, the colonies with the characteristic double halo of hemolysis were re-streaked onto blood agar medium for purification. After 24 h catalase test and gram staining were done. Culture on egg yolk agar, SIM and lithmus milk under anaerobic conditions confirmsthe presence of C. perfringensis. PCR assays have been established to genotype C. perfringensis with respect to the genes cpa, cbp, etx, and iap, encoding the alpha, beta, epsilon and iota toxins, respectively. DNA from the thawed suspension was prepared with the Easy DNA kit according to the manufacturer’s instructions and the multiplex PCR was done. PCR products were subjected to electrophoresis for 120 min at 80 V. Amplified bands were visualized and photographed under UV illumination.
Results: Based on bacterial culture and toxinotyping of the isolate this case was diagnosed as enterotoxemia by C. perfringens type A.
Conclusion: It was concluded from the present study that enterotoxaemia can occur in gazelle with clinicalsigns and pathological lesions similar to those of other small ruminants.

 
Keyword(s): CLOSTRIDIUM PERFRINGENS, GAZELLE, PCR
 
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