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Paper Information

Journal:   IRANIAN JOURNAL OF PUBLIC HEALTH   AUGUST 2014 , Volume 43 , Number SUPPLEMENT 2; Page(s) 64 To 64.
 
Paper: 

CHARACTERIZATION OF CHLAMYDIA TRACHOMATIS OMP1 GENOTYPES ISOLATED FROM PATIENTS WITH FOLLICULAR CONJUNCTIVITIS REFERRED TO FARABI HOSPITAL BY RFLP

 
 
Author(s):  ABEDINIFAR ZOHREH*, FALLAH FATEMEH, ASADIAMOLI FAHIMEH, DOUSTDAR FARAHNOOSH, GOUDARZI HOSSEIN
 
* DEPARTMENT OF MICROBIOLOGY, FACULTY OF MEDICINE, SHAHID BEHESHTI UNIVERSITY OF MEDICAL SCIENCES, TEHRAN, IRAN
 
Abstract: 

Background: In this study, we used Omp1 Genotyping for analysis of samples from patients with follicular conjunctivitis referred to Farabi Hospital.
Methods: Conjunctival scraps (n=90) were obtained from patients who were referred to the diagnostic laboratory of Farabi hospital during 2012. Smears were prepared by rolling half the swab on to the center of a glass slide. Smears were fixed and used for direct immunofluorescence (DIF) method using a genus specific fluorescein isothyocyanate labelled Chlamydia monoclonal antibody. In Giemsa stain, diagnosis was based on the prescience of inclusions that were basophilic and stained pinkish –blue. PCR amplification after extraction was performed using CT1 and CT5 primers designed from Omp1 gene. Omp1 Genotyping was performed by RFLP method using AluI.
Results: Among 90 cases examined for Chlamydia trachomatis in eyes, 28 patients (31.1%) were positive by DIF and 13 (14.4%) by Giemsa staining and 35 (38.8%) showed positive result in PCR. C. trachomatis genotypes E (51/2%), G (21/9%), I (14.6%) and F (4.8%) were the most prevalent serovars among patients with follicular conjunctivitis.
Conclusion: In our comparison, PCR detected ocular C. trachomatis infection significantly more often than DIF and Giemsa stain. The results of this study show that PCR has higher sensitivity and sensitivity comparing DIF and Giemsa stain. So it is proposed that PCR is used as a conventional method for detecting chlamydial infections in eye. Urogenital serovars were the most prevalent serovars among patients with follicular conjunctivitis.

 
Keyword(s): FOLLICULAR CONJUNCTIVITIS, CHLAMYDIA TRACHOMATIS, OMP1 (OUTER MEMBRANE PROTEIN)
 
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