Paper Information

Journal:   IRANIAN JOURNAL OF CHEMISTRY AND CHEMICAL ENGINEERING (IJCCE)   2014 , Volume 33 , Number 2; Page(s) 93 To 98.
 
Paper: 

LIPOXYGENASE-1 LEACHING FROM SOYBEAN FLOUR EMPLOYING STIRRED TANK CONTACTOR

 
 
Author(s):  ROSTAMI KHOSROW*, DAYANI YASAMAN, ESFAHANI BOLANDBALAIE ZAHRA, RASHTCHIAN DAVOOD
 
* DEPARTMENT OF BIOTECHNOLOGY, IRANIAN RESEARCH ORGANIZATION FOR SCIENCE AND TECHNOLOGY, TEHRAN, I.R. IRAN
 
Abstract: 

The present work examines leaching of lipoxygenase-1 from soybean flour employing stirred tank vessel. The effect of operating parameters, impellers speed, operational period, temperature, pH and scale-up were considered. The acetic acid pH: 5.2 increased the leaching of lipoxygenase- 1. The sensitivity of agitator speed and geometrical-scale-up on the enzyme leaching has been conducted. The effect of agitator speed on the geometrically-scaled- up reactor has shown that similar amount of enzyme is leached at lower speed. The result is in para with the conventional system. The importance of operational period from 5 to 50 min on the enzyme leaching was evaluated and higher enzyme leaching obtained within 10 min. of operation. The Sigmaplot-6 for statistical verification and developing a correlation for the enzyme leaching was used.

 
Keyword(s): ENZYME LEACHING, LIPOOXYGENASE-1, OPERATIONAL PERIOD, SOYBEAN FLOUR, STIRRED TANK CONTACTOR
 
References: 
 
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