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Paper Information

Journal:   IRANIAN JOURNAL OF PUBLIC HEALTH   AUGUST 2014 , Volume 43 , Number SUPPLEMENT 2; Page(s) 62 To 62.
 
Paper: 

THE PREVALENCE OF CHLAMYDIA TRACHOMATIS IN PATIENTS WITH BENIGN AND MALIGNANT OVARIAN CANCER BY NESTED PCR METHOD

 
 
Author(s):  DADASHI MASOUD*, ESLAMI GITA, GHALAVAND ZOHREH, TAHERPOUR AREZOU, GOUDARZI HOSSEIN, FALLAH FATEMEH, ZAHIRNIA ZAHRA
 
* DEPARTMENT OF MICROBIOLOGY, SCHOOL OF MEDICINE, SHAHID BEHESHTI UNIVERSITY OF MEDICAL SCIENCES, TEHRAN, IRAN
 
Abstract: 

Background: The aim of this study was to investigate the prevalence of C.trachomatis in patients with ovarian cancer that referred to Imam Hossein Hospital in Tehran, Iran.
Methods: In this descriptive study that was conducted from January 2013 to April 2014, 120 pathological samples were studied which obtained from patients with ovarian cancer who referred to Imam Hossein Hospital. The samples were transferred to the laboratory of Shahid Beheshti University of Medical Sciences. DNA was extracted with QIAamp blood mini kit (Qiagen, USA) according to manufacturer's instructions. In order to detect the presence of C.trachomatis, specific primers for the MOMP (Major Outer Membrane Protein) genes of C.trachomatis were designed and evaluated by Nested PCR method.
Results: Out of 120 samples of ovarian cancer, 60 (50%) samples were malignant cancer and 60 (50%) were benign cancer as control group. Results of Nested PCR showed that 14 (23.3%) malignant samples were positive for the presence of C. trachomatis. None of the tissue samples of ovarian benign cancer was positive for C. trachomatis.
Conclusion: Our results suggest that the spread of C.trachomatis in the female with ovarian cancer may be common. This finding reflects a possible role of C.trachomatis in the carcinogenesis of ovarian tumors. C.trachomatis infection may play a relative role in the pathogenesis of ovarian carcinomas or it could facilitate its progression.

 
Keyword(s): CHLAMYDIA TRACHOMATIS, OVARIAN CANCER, NESTED PCR
 
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