Click for new scientific resources and news about Corona[COVID-19]

Paper Information

Journal:   IRANIAN JOURNAL OF PUBLIC HEALTH   AUGUST 2014 , Volume 43 , Number SUPPLEMENT 2; Page(s) 62 To 62.
 
Paper: 

MYCOPLASMA CONTAMINATION IN CELL CULTURES TREATED WITH CIPROFLOXACIN AND ENROFLOXACIN

 
 
Author(s):  SOLTANIAN BITA*, IRANI SHIVA, HASHEMI SARVENAZ, CHERAGHI YUSEF, GHOLAMI ALI REZA
 
* DEPARTMENT OF BIOLOGY, FACULTY OF SCIENCES, ISLAMIC AZAD UNIVERSITY SCIENCE AND RESEARCH
 
Abstract: 

Background: In this study, Mycoplasma -infected cell lines treatment was done by different dilutions of ciprofloxacin and enrofloxacin that inhibits DNA replication and protein synthesis, treatment and removal of Mycoplasma contamination in cell cultures infected verified by PCR method.
Methods: Different dilutions of antibiotics such as Ciprofloxacin and Enrofloxacin were used in sequential passages for treatment of the infected Vero cell. Antibiotic treatment with ciprofloxacin and enrofloxacin in contaminated cell to eliminating Mycoplasma relying on the lowest passage was compared, the result of removal Mycoplasma by antibiotics were determined by PCR method in each step.
Results: Most efficient concentration of Ciprofloxacin and Enrofloxacin was suggested by topsis method, the dilution of Ciprofloxacin and Enrofloxacin and ability of them in removing Mycoplasma and also the time of treatment were compared and verified.
Conclusion: Proposed concentration of ciprofloxacin is 60 ?g/ml, and in the second order it is 30
mg/ml, for Enrofloxacin. The best-proposed effective concentrations respectively are 30, 300 and 3 mg/ml dilutions. In the present method, treatment with antibiotics was done without any shifting inantibiotic's family.

 
Keyword(s): MYCOPLASMA, CELL CULTURE, CIPROFLOXACIN, ENROFLOXACIN, PCR
 
References: 
  • ندارد
 
  Yearly Visit 64
 
Latest on Blog
Enter SID Blog