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Paper Information

Journal:   IRANIAN JOURNAL OF PUBLIC HEALTH   AUGUST 2014 , Volume 43 , Number SUPPLEMENT 2; Page(s) 60 To 60.
 
Paper: 

SCREENING FOR URICASE ENZYME FROM HALOTOLERANT BACTERIA

 
 
Author(s):  HONARBAKHSH FATEMEH*, AMOOZEGAR MOHAMMAD ALI, ABOLMAALI SHAMSOZOHA, MEHRSHAD MALIHEH
 
* DEP. OF MICROBIAL BIOTECHNOLOGY FACULTY OF BIOTECHNOLOGY, SEMNAN UNIVERSITY, SEMNAN, I.R., IRAN
 
Abstract: 

Background: Uricase (urate oxidase, EC 1.7.3.3) is the enzyme that catalyzes specifically the oxidation of uric acid to allantoin and plays an important role in nitrogen metabolism. Uric acid and its salts are relatively insoluble in water, easily precipitated, and the abnormal accumulation of uric acid is a causative factor of gout in humans. The aim of this study was screeinning for new resources for Uri case; to be most effective as well as commercially valuable.
Methods: All strains investigated in this study isolated from different saline and hyper saline lakes and wetlands from Iran. A total of 65 halo tolerant strains were tested in this project. Strains were cultured on a production medium containing 0.3% uric acid and total salt 3%. The agar plates were incubated at 30 ? C and monitored 48 h. A clear zone indicates the presence of uricase enzyme. Strains with clear zone were selected for quantitative assay with UV visible-spectrophotometer at 293nm. Decrease of absorbance indicated elimination of uric acid by uricase activity.
Results: Our results revealed some species of Halomonas and Paracoccus with high enzyme activity which can be used as a new resource for production of uricase.
Conclusion: This investigation showed that the halotolerant bacteria are potentially resources for uricase under stress condition with a commercially valuable quality and quantities.

 
Keyword(s): URICASE, HALOTOLERANT BACTERIA, SCREENING
 
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