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Paper Information

Journal:   IRANIAN JOURNAL OF PUBLIC HEALTH   AUGUST 2014 , Volume 43 , Number SUPPLEMENT 2; Page(s) 60 To 60.
 
Paper: 

EFFECTS OF BUFFER ON THE KINETIC BEHAVIOUR OF BACTERIAL ENZYME: XANTHAN LYASE

 
 
Author(s):  EBRAHIMI MARYAM*, MOOSAVI NEJAD ZAHRA
 
* ALZAHRA UNIVERSITY, TEHRAN, IRAN
 
Abstract: 

Background: The aim of this study isthe investigation of type of buffer (as an environmental factor) on xanthan lyase activity, a xanthan-degrading enzyme from Paeni Bacillus SP.SM0.
Methods: In the present study, bacterial xanthan lyase was cultured in fluid culture media. Supernatant was collected and activity of the enzyme was measured. Xanthan lyase activity was monitored by measuring the increase of A235 caused by conjugation of the formed C=C bond with the carboxylate group in the uronic acid residue.
Results: The enzyme saturation curve (Michaelis-Menten plot) was plotted in increasing concentrations of xanthan (substrate) in the presence of both phosphate and tris-HCl buffer. Vmax and Km of the enzyme were measured and comprised.
Conclusion: According to the parameters, it can be deduced that buffer has an effect on xanthan lyse activity and tris-HCl buffer is more suitable for a better activity of xanthan lyase.

 
Keyword(s): POLYSACCHARIDE, XANTHAN LYSE ACTIVITY, BUFFER
 
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