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Paper Information

Journal:   IRANIAN JOURNAL OF PUBLIC HEALTH   AUGUST 2014 , Volume 43 , Number SUPPLEMENT 2; Page(s) 58 To 58.
 
Paper: 

EVALUATION OF MICROBIAL LIFE IN SALT LAKE RAZAZEH, KARBALA – IRAQ

 
 
Author(s):  AL RUBAYE MUSHTAQ TALIB SHAWI*, HOSSEINI MARYAM, FAKHARI JAVAD, JABBER AL MUSAWI MASTAFA HEILO
 
* DEPARTMENT OF GENETIC, FACULTY OF BIOLOGY, SHAHID BEHESHTI UNIVERSITY, TEHRAN, IRAN
 
Abstract: 

Background: The aim of this research was to evaluate halophilic bacterial diversity in Lake Razazeh as regards this is the first report on halophilic bacteria isolation in Iraq.
Methods: Samples of saline soils, mud and water were collected from 15 locations of Lake Razazeh with distance of 4 km from each other. Halophiles were isolated by saline nutrient broth enrichment. The growth was diluted 10 times and plated on complete medium agar with a salt gradient of 2.5 percent. Cultures were incubated at pH 7-7.5 and 37oC during 7 days given that all medium were controlled every day. Gram staining was carried out as a routine initial procedure in the identification of unknown bacterial species.
Results: Two hundred and eighteen isolates were selected from isolation plates, which were named K1-K218. There were 161 gram-positive rod and cocci and 57 gram-negative rods. More than fifty strains were isolated using the morphological differences based on visible examination of the growth characteristics, and were used for further analysis. Also, 15 protozoa, 3 algae, 5 fungi and yeast were observed in the culture medium. It was found that the bacteria diversity in different media varied considerably so that only one type of bacteria grow in the 3 medium which likely showed these three bacteria are antibiotic-containing. About two third of the isolates were halotolerant and only eighteen isolates grew at NaCl concentrations greater than 17.5%.
Conclusion: The data presented here show that despite drought, dehydration, increased concentrations of salts and contaminants, Lake Razazeh near Karbala represents an untapped source of halophilic bacteria biodiversity.

 
Keyword(s): BIODIVERSITY, HALOPHILES, SCREENING
 
 
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APA: Copy

AL RUBAYE, M., & HOSSEINI, M., & FAKHARI, J., & JABBER AL MUSAWI, M. (2014). EVALUATION OF MICROBIAL LIFE IN SALT LAKE RAZAZEH, KARBALA – IRAQ. IRANIAN JOURNAL OF PUBLIC HEALTH, 43(SUPPLEMENT 2), 58-58. https://www.sid.ir/en/journal/ViewPaper.aspx?id=410334



Vancouver: Copy

AL RUBAYE MUSHTAQ TALIB SHAWI, HOSSEINI MARYAM, FAKHARI JAVAD, JABBER AL MUSAWI MASTAFA HEILO. EVALUATION OF MICROBIAL LIFE IN SALT LAKE RAZAZEH, KARBALA – IRAQ. IRANIAN JOURNAL OF PUBLIC HEALTH. 2014 [cited 2021November27];43(SUPPLEMENT 2):58-58. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=410334



IEEE: Copy

AL RUBAYE, M., HOSSEINI, M., FAKHARI, J., JABBER AL MUSAWI, M., 2014. EVALUATION OF MICROBIAL LIFE IN SALT LAKE RAZAZEH, KARBALA – IRAQ. IRANIAN JOURNAL OF PUBLIC HEALTH, [online] 43(SUPPLEMENT 2), pp.58-58. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=410334.



 
 
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