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Paper Information

Journal:   IRANIAN JOURNAL OF PUBLIC HEALTH   AUGUST 2014 , Volume 43 , Number SUPPLEMENT 2; Page(s) 55 To 55.
 
Paper: 

CONTINUOUS SCREENING OF LIPID DEGRADATING YEASTS AND ITS APPLICATION IN TREATMENT OF OILY WASTEWATER

 
 
Author(s):  FARAZANDEH MEHR SHIMA*, HAMEDI JAVAD, MOGHIMI HAMID, TABATABAEI MEYSAM
 
* MICROBIAL TECHNOLOGY AND PRODUCTS RESEARCH CENTER, DEPARTMENT OF MICROBIAL BIOTECHNOLOGY, SCHOOL OF BIOLOGY, COLLEGE OF SCIENCE, UNIVERSITY OF TEHRAN, TEHRAN, IRAN
 
Abstract: 

Background: The aim of this study was to continue the screening of lipophilic yeasts with high specific growth rate for wastewater treatment.
Methods: Soil samples were collected from soil with oil pollution. These samples were used in a continuous screening system in which fresh medium was poured in the main container as feed and the waste was removed in periodically time scale. Samples were taken from the main container each day and cultured in PDA plus chloramphenicol (100 mg/L) and Rose Bengal. A synthetic wastewater containing 10 g of olive oil, 0.6 g of peptone, 0.4 g of beef extract, 0.1 g of urea, 0.1 g of Na2HPO4, 0.03 g of NaCl, 0.014 g of CaCl2, 0.014 g of KCl, and 0.01 g of MgSO4 per liter was used as the representative of lipid containing wastewater. This medium was incubated at 28oC in rotary shakers and the lipid degradation rate was investigated with standard n-hexan oil extraction method for 3 days.
Results: In this study, continuous screening was used to isolate the most predominant yeast capable of degradating lipids with high efficiency and growth rate. The obtained isolate was identified by 18S rRNA gene sequence analysis as Rhodotorula mucilaginosa (UTMC 5004). The entire isolates were preserved in University of Tehran Microorganisms Collection (UTMC). The oil degradation of the R. mucilaginosa reached 67 % in 3 days with 6.8 % wet cell weight.
Conclusion: Although lipid-degradading bacteria have been examined exceedingly, yeasts have been less studied in terms of their great potentials in degrading lipids. These results indicate that R. mucilaginosa UTMC 5004 can be used in biological treatment of lipid containing wastewater and can be further investigated for the optimization of the process.

 
Keyword(s): LIPID DEGRADATION, RHODOTORULA MUCILAGINOSA, CONTINUOUS SCREENING, WASTEWATER TREATMENT
 
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