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Paper Information

Journal:   IRANIAN JOURNAL OF PUBLIC HEALTH   AUGUST 2014 , Volume 43 , Number SUPPLEMENT 2; Page(s) 54 To 54.
 
Paper: 

INVESTIGATION OF DIFFERENT MICROALGAE CELL DISRUPTION METHODS

 
 
Author(s):  MIRSHEKARI SOLEIMAN, ARABIAN DARYUSH*, KHALILZADEH RASOUL, ABASPOUR FARZIN
 
* DEPARTMENT OF BIOSCIENCE AND BIOTECHNOLOGY, MALEK ASHTAR UNIVERSITY OF TECHNOLOGY, IRAN
 
Abstract: 

Background: Cell disruption processes are important pretreatment techniques for extraction of intracellular contents from microalgae. In this study we tried to compare the results obtained for different cell disruptions methods by calculating the total chlorophyll content.
Methods: We have evaluated six different methods; ultrasonic, osmotic shock, microwave, bead mill, liquid nitrogen and centrifuges as the cell disruption methods. 0.2 grams of dry powders of Chlorella vulgaris are mixed with 30 ml 90% methanol and after cell disruption, samples were kept at -20?c for 24 hours. Finally total chlorophyll content was calculated according to the data obtained.
Results: By comparing the results obtained for the total chlorophyll contentrespectively ultrasonic methods (7.50 mg/l), bead mill (6.38 mg/l), osmotic shock (4.50 mg/l), microwave (4.11 mg/l), centrifuges (3.21 mg/l) and liquid nitrogen (3.01 mg/l) had the best performance in the destruction of the cell wall.
Conclusion: Although ultrasound is the highest efficient in the disruption of cell wall, but this method and microwave due to the high cost of energy consumption can be used only in laboratory studies and osmotic shock due to excessive time required (48 hr) is not appropriate. In industrial scale, bead mill due to high efficiency and less cost of energy consumption is suggested.

 
Keyword(s): CHLOROPHYLL, CELL DISRUPTION, MICROALGAE
 
References: 
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