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Paper Information

Journal:   IRANIAN JOURNAL OF PUBLIC HEALTH   AUGUST 2014 , Volume 43 , Number SUPPLEMENT 2; Page(s) 54 To 54.
 
Paper: 

QUANTITATIVE ASSAY OF ANTITUMOR ENZYMES FROM HALOPHILIC AND HALOTOLERANT BACTERIA

 
 
Author(s):  ZOLFAGHAR MAHDIS*, AMOOZEGAR MOHAMMAD ALI, KHAJEH KHOSRO
 
* EXTREMOPHILES LAB., DEPT. OF MICROBIOLOGY, FACULTY OF BIOLOGY, COLLEGE OF SCIENCE, UNIVERSITY OF TEHRAN, TEHRAN, IRAN
 
Abstract: 

Background: Halophilic bacteria may contain antitumor enzymes with novel immunological properties that can be used in hypersensitive patients.
Methods: In former investigation, qualitative screening for L-asparaginase, L-Glutaminase and L-Arginase had been done. In the next step, enzymes activity was measured during growth curve plotting of positive strains. For this purpose, 1.5 ml of each positive strain inoculum (0.5 McFarland standards) was added to 50ml broth medium (supplemented with 2.5% total salts and substrate) and incubated in shaker incubator (150 rpm, 34oC). During their growth curve plotting, quantitave assay of the enzymes was measured by Nessler’s reagent (For L-glutaminase and L-asparaginase) and ninhydrine (For L-arginase).
Results: Among 115 strains, 33, 7 and 5 strains were positive in L-asparaginase, L-glutaminase and L-arginase production, respectively. Activity of all the enzymes was increased during logarithm phase and maximum activity (IU/ml) was observed in stationary phase, especially in the end of stationary phase. Bacillus sp. strain R2S25 (7.5 IU/ml), Bacillus sp. Strain R2S12 (0.27 IU/ml) and Planococcus sp. Strain GAAy3 (5.5 IU/ml) respectively demonstrated maximum activity in Lasparaginase, L-gltaminase and L-arginase, respectively. 1 I.U (International Unit) of the enzyme is equal to amount (Tl, pmol) of product produced from enzyme per minute.
Conclusion: This investigation showed that the halophilic and halotolerant bacteria from different hypersaline environments in Iran are a potential source of antitumor enzymes and may have possess commercial value.

 
Keyword(s): ANTITUMOR ENZYME, HALOPHILIC BACTERIA, LASPARAGINASE, L-GLUTAMINASE, L-ARGINASE
 
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