Paper Information

Journal:   IRANIAN JOURNAL OF PUBLIC HEALTH   AUGUST 2014 , Volume 43 , Number SUPPLEMENT 2; Page(s) 53 To 53.
 
Paper: 

STUDY OF ORGANOPHOSPHORUS INSECTICIDE CHLORPYRIFOS BIODEGRADATION BY HALOTOLERANT BACTERIA ISOLATED FROM CONTAMINATED AGRICULTURAL LANDS

 
 
Author(s):  RAFIEYAN SHOKUFEH*, AMOOZEGAR MOHAMMAD ALI, SHAVANDI MAHMOUD, MEHRNIA MOHAMMAD REZA
 
* EXTREMOPHILE LAB., DEPT. OF MICROBIOLOGY, FACULTY OF BIOLOGY, COLLEGE OF SCIENCE, UNIVERSITY OF TEHRAN, TEHRAN, IRAN
 
Abstract: 

Background: Because many agricultural lands in Iran are saline, aim of this project is to investigate the biodegradation of cloropyrifos as an Organophosphorus (OP) compound model by halo-tolerant bacteria isolated from agricultural land.
Methods: Soil and water samples were collected from saline agricultural areas with history use of OP. For isolation of bacteria capable of utilizing CP as sole carbon source, enrichment technique with the modified-M9 medium supplemented with 50 ppm CP and 5% NaCl was used. The time of each round of enrichment was 14 days. After three rounds of enrichment, modified R2A agar medium (with 5% NaCl) was used for isolation and purification of single colonies. To select the isolates with higher resistance to CP toxicity and CP-degraders that are more efficient the growth of selected strains in modified-M9 media containing 100 to 500 ppm CP as sole source of carbon was assayed after 7 days with UVvisible spectrophotometer at 600nm. Plate-count method was used to compare the growth rate of the isolates in presence of 500 ppm CP.
Results: After three rounds of enrichment, 28 morphologically different bacterial colonies were isolated and purified. Evaluation of the growth of the isolates in the presence of 100 ppm CP after 7 days, led to selection of 5 isolates for further studies. Growth measurement of the selected strains in presence of 100 to 500 ppm CP revealed that the rate and extent of the growth of 3 strains CDB1, CDB2 and CDB3 have increased along with pesticide concentration, the results of plate count method also confirmed the increased growth of isolates in 500 ppm CP. Microscopic examination of these isolates indicated that all of the strains are Gram-negative bacilli.
Conclusion: Bacteria isolated in this study which are able to use CP as the sole carbon source for their growth, can be promising candidates to remove these compounds from contaminated brackish water bodies and because of structural similarity of OP pesticides, they can be helpful for biodegradation of other members.

 
Keyword(s): ORGANOPHOSPHORUS (OP) PESTICIDE, CHLOROPYRIFOS, BIODEGRADATION, HALOTOLERANT BACTERIA
 
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