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Paper Information

Journal:   IRANIAN JOURNAL OF PUBLIC HEALTH   AUGUST 2014 , Volume 43 , Number SUPPLEMENT 2; Page(s) 52 To 52.
 
Paper: 

NUTRIENT AND PHYSICAL VARIABLES OPTIMIZATION OF PHB PRODUCTION BY ALCALIGENES EUTROPHUS

 
 
Author(s):  DASHTI AGJEH SAEID, ARAMVASH ASIEH, AKBARI SHAHABI ZEINAB, GHAFARI MOHAMMAD DAVOOD
 
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Abstract: 

Background: Polyhydroxyalkanoates (PHAs) are completely biodegradable thermoplastics, which are accumulated as an energy reserve material by a large number of bacteria in the presence of excess carbon source and limitation of nitrogen or phosphorous sources. In the present study, efforts have been made to optimize the effect of various nutritional and physical parameters for enhanced PHB production by Alcaligenes eutrophus PTCC 1615 using Plackett-Burman and Response Surface Methodology statistical methods.
Methods: In the present study, efforts have been made to optimize the effect of various nutritional and physical parameters for enhanced PHB production by Alcaligenes eutrophus PTCC 1615 using Plackett-Burman and Response Surface Methodology statistical methods. At first, based on onefactor- at-a-time experiments, fructose and ammonium chloride were found to be the most suitable sources of carbon and nitrogen for PHB production.
Results: Then through the Plackett-Burman and central composite design fructose, agitation speed, KH2PO4, and initial pH were recognized as the most significant factors affecting PHB accumulation. ANOVA analysis of the model showed a significant interaction between fructose and agitation speed. Conclusion: After optimization of the medium, compositions for PHB production were determined as: fructose 45 g/L, KH2PO4 2.12 g/L, MgSO4.7H2O 1.2 g/L, citric acid 1.7 g/L, trace element 10 mL/L, initial pH=7.78 and agitation speed 198 rpm. Under this optimal culture conditions, the maximum yield of PHB was 9.41 g/L. These results are the highest values of PHB ever obtained from A. eutrophus reported so far.

 
Keyword(s): BIOPOLYMERS, PHA, BIODEGRADABLE, ALCALIGENES EUTROPHUS
 
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