Paper Information

Journal:   IRANIAN JOURNAL OF PUBLIC HEALTH   AUGUST 2014 , Volume 43 , Number SUPPLEMENT 2; Page(s) 52 To 52.
 
Paper: 

RHAMNOLIPID PRODUCTON USING SOYBEEN OIL REFINERY WASTES

 
 
Author(s):  PARTOVI MARYAM*, BAGHERI LOTFABAD TAYEBE, HADDADI AZAM
 
* DEPARTMENT OF MICROBIOLOGY, KARAJ BRANCH ISLAMIC AZAD UNIVERSITY, KARAJ, IRAN
 
Abstract: 

Background: Rhamnolipid type biosurfactant production by Pseudomonas aeruginosa MR01 was investigated using soapstok waste of soybeen oil refinery as a Low Cost carbon source. Physiochemical properties of rhamnoipid MR01 were studied to figure out the possibility of using this product in idustrial aplications.
Methods: In this research, the production yield of P. aeruginosa MR01 was investigated in different broths, each containing 2%, 4%, 6%, 8%, 10% and 12% (V/V) of soapstock. The production was purified using column chromatography. Biosurfactant emulsification was determined through the emulsification index (E24). And critical micelle concentration was measured by ring method.
Results: The best yield was obtained using 8% V/V of soapstock. The purity of the production was 89.28%. The MR01 rhamnolipids from soapstock showed good tensioactive properties with a minimal surface tension of 26.34 mN/m and a critical micelle concentration (CMC) of 59.25 mg/l. The MR01 biosurfactants formed stable emulsions with Kerosene and showed excellent emulsification of this substance (66.66%).
Conclusion: These results demonstrate the possibility of using soapstock as an excellent carbon source and the production can be useful for industrial applications, such as the bioremediation of oil spills.

 
Keyword(s): RHAMNOLIPID, BIOSURFACTANT, PSEUDOMONAS AERUGINOSA
 
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