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Paper Information

Journal:   ARCHIVES OF PEDIATRIC INFECTIOUS DISEASES   OCTOBER 2014 , Volume 2 , Number 4; Page(s) 1 To 5.
 
Paper: 

ESTIMATION OF GROUP B STREPTOCOCCUS COLONIZATION IN HIGH-RISK NEONATES BY PCR AND STANDARD CULTURE

 
 
Author(s):  SHIRVANI FARIBA*, SHAHROCHI NADER, RADFAR MITRA, LAKESTANI DAVOOD
 
* PEDIATRIC INFECTIONS RESEARCH CENTER, MOFID CHILDREN’S HOSPITAL, SHAHID BEHESHTI UNIVERSITY OF MEDICAL SCIENCES, TEHRAN, IR IRAN
 
Abstract: 

Background: Group B Streptococcus (GBS) (Streptococcus agalactiae) is the leading cause of morbidity and mortality of newborn infants considered a leading factor causing septicemia after birth. The standard method for the diagnosis of GBS colonization is culture in a selective medium, but PCR has a high sensitivity and specificity.
Objectives: The goal of this study was to estimate the colonization of GBS in rectum of neonates of high-risk mothers by culture and PCR method.
Patients and Methods: Samples were taken from rectal mucosa of 154 neonatesof high-risk mothers for GBS by swabs. Samples were tested by standard culture using Todd Hewitt broth and blood agar and also by PCR using primers specific for cfb gene.
Results: Of 154 neonates, Culture identified 17 (11%) neonates as colonized by GBS, and the PCR assay could identify 27 (17%) neonates with positive results for GBS. Mothers age range was 17-40 years (mean=26.1
±5.1). Maternal age was significantly lower in PCR positive group (P=0.038) and in culture positive group (P=0.015). Using culture as the gold standard, sensitivity, NPV, specificity, and PPV of PCR were 100%, 100%, 92%, and 62%, respectively. The time required for PCR assay and culture were 2hours and 36hours, respectively.
Conclusions: This study showed that the incidence of GBS in Iranian high-risk neonates is high, so we strongly recommend screening of high-risk neonates for detection of GBS.

 
Keyword(s): STREPTOCOCCUS AGALACTIAE, INFANT, NEWBORN, COLONIZATION
 
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