Paper Information

Journal:   IRANIAN JOURNAL OF PUBLIC HEALTH   AUGUST 2014 , Volume 43 , Number SUPPLEMENT 2; Page(s) 49 To 49.
 
Paper: 

ISOLATION OF PHYTASE-PRODUCING BACTERIA FROM RHIZOSPHERE OF IRANIAN WHEAT

 
 
Author(s):  FAYAZI HOSSEINI NASHMIN*, FOOLADI JAMSHID, KIAROSTAMI KHADIJEH
 
* DEPARTMENT OF BIOLOGY, FACULTY OF BASIC SCIENCES, ALZAHRA UNIVERSITY, TEHRAN, IRAN
 
Abstract: 

Background: In this study, phytase-producing bacteria from7 cultivar of rhizosphere wheat (Chamran, Tajan, Morvarid, Pishtaz, Oroum, Sivand, Zarea) were solated and identified based on morphological and biochemical characteristics.
Methods: Enzyme-producing bacteria were determined by production of clear zones around the colonies on the medium containing sodium phytate, after 24-48 h of incubation at 30oC. Because some bacteria in phytase specific medium are able to create false positive results, we eliminated them by phytase plate assay (cobalt chloride, ammonium molybdate / ammonium vanadate). The best phytase producing isolates were recognized by its surrounding clear halo.
Results: Out of 47 colonies on media plates, 19 colonies showed positive for phytase production and created zone of clearing around microbial colonies, but 11 false positive colonies were detected. Finally, 8 isolates were detected as real phytase-producing bacteria. Most isolated bacteria belong to two cultivars (Tajan, Chamran). One strain creating 23 mm zone was selected as the best enzyme producing isolate.
Conclusion: This research provided important data about phytase producing bacteria isolated from rhizosphere wheat, which will facilitate future research on the optimization of fermentatoin processes for production of high phytase activity.

 
Keyword(s): PHYTINE, PHYTASE, PHOSPHATASE, RHIZOSPHERE WHEAT, CULTIVAR
 
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