Background: The purpose of the present study was investigation of amylase production using black sugar by submerged fermentation method and process optimization by members of the genus Bacillus.
Methods: Optimization of different factors such as time, pH, shaking, and inoculation of amylase enzyme on production in submerged culture using RSM method were conducted.
Results: The maximum amount of production of experimental enzyme obtained in submerged culture at 24 hours, level of rpm 250, and pH=6, and inoculation rate of 20% was achieved.
Conclusion: Since black sugar is native for guilan province, and also in terms of price is affordable, it has been used in this study.