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Paper Information

Journal:   IRANIAN JOURNAL OF PUBLIC HEALTH   AUGUST 2014 , Volume 43 , Number SUPPLEMENT 2; Page(s) 48 To 48.
 
Paper: 

EVALUATION OF CRUDE OIL BIODEGRADATION AND LACCASE ACTIVITY IN NEWLY ISOLATED PHAEOSPHAERIA SP UTMC 5003

 
 
Author(s):  HEIDARY TABAR REZVAN*, MIGHMI HAMID, HAMEDI JAVAD
 
* DEPARTMENT OF MICROBIAL BIOTECHNOLOGY, SCHOOL OF BIOLOGY AND CENTER OF EXCELLENCE IN PHYLOGENY OF LIVING ORGANISMS, COLLEGE OF SCIENCE, UNIVERSITY OF TEHRAN, TEHRAN, IRAN
 
Abstract: 

Background: The present study was carried out to investigate Iranian indigenous fungal strains to remove crude oil pollutants.
Methods: First, for the fungal strain isolation, the contaminated soil samples were collected from different habitats of Iran. The soil samples were enriched in Minimal Salt Medium (MSM) supplemented with 1% crude oil, and chloramphenicol. Purification of isolates was carried out on PDA media. Afterward, each of pure fungal isolates were evaluated for growth rate and degradation ability after 15 days under 1% crude oil in MSM, by measuring the dry weight, total petroleum hydrocarbons (TPH) assay at 420 nm and the residual hydrocarbon content by FTIR. In the following, laccase activity of this isolate in the presence of 1% crude oil was measured. Finally, the best isolate was identified based on 18s rRNA gene sequence analysis and its morphology of colony and microscopic examination with the reference to identification keys.
Results: 30 different fungal strains were isolated. TPH test and gravimetric growth of each isolate in MSM supplemented with 1% crude oil as the sole carbon source showed that the isolate G-05 with 65% degradation over 15 days, was the best isolate in removing the petroleum hydrocarbons. Residual crude oil analysis with FTIR spectrophotometry in PDA+1% crude oil medium also indicated that G-05 is able to degrade 90% of aliphatic compounds. Tensiometric assay showed that G-20 is a potent strain for bio-surfactant production. Evaluation of laccase activity showed that this isolate can produced 1440 U/l of enzyme at the end of 15 days. Molecular and morphological identification indicated that G-05 is Phaeosphaeria spp.
Conclusion: In spite of high potential of fungi in bioremediation of contaminants such as crude oil pollutants, few studies have been carried out in this field. The results of this study showed that this isolate that is being reported for the first time, has a high potential in bioremediation of the soils contaminated oil.

 
Keyword(s): BIOREMEDIATION, PHAEOSPHAERIA SPP, CRUDE OIL, ENVIRONMENTAL BIOTECHNOLOGY
 
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