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Paper Information

Journal:   IRANIAN JOURNAL OF PUBLIC HEALTH   AUGUST 2014 , Volume 43 , Number SUPPLEMENT 2; Page(s) 48 To 48.
 
Paper: 

ISOLATION AND CHARACTERIZATION OF PHENOL- DEGRADING BACTERIA FROM THE ARAK PETROCHEMICAL COMPLEX

 
 
Author(s):  SAFAEI NASIM*, EBRAHIMIPOUR GHOLAMHOSSEIN, SHAHRIARI MOGHADAM MOHSEN
 
* DEPARTMENT OF MICROBIOLOGY, FACULTY OF BIOLOGICAL SCIENCE, SHAHID BEHESHTI UNIVERSITY, TEHRAN, IRAN
 
Abstract: 

Background: Phenol is an environmental pollutant present in industrial wastewaters such as refineries, coal processing and petrochemicals industries. Bioremediation has become one of the most rapidly developing fields of environmental restoration, utilizing microorganisms to reduce the concentration and toxicity of various chemical pollutants. This approach represents a good alternative compared with physicochemical strategies, which have high costs and produce other toxic products.
Methods: Phenol degrading bacteria were isolated from activated sludge of Arak Petrochemical Complex by serial dilutions of enriched consortium. In order to assess the utilization rates of phenol using the isolated strains, microbial inoculums were passed to the mineral salt medium with phenol (1000 mg/l) as the sole carbon and energy source. The efficiency of isolated strains was assessed by monitoring the bacterial growth (OD600nm) and phenol biodegradation (spectrometric ally using 4-aminoantipyrene). The experimental cultures were performed at 30oC at 140 rpm for 7 days.
Results: Among the isolated strains, NS1 had the highest rate of growth. After 7 days its degradation was approximately 70%.This strain was round-shaped, forming small flat orange colonies (on nutrient agar medium), gram positive, oxidase negative and catalase positive.
Conclusion: Isolated bacteria from Arak Petrochemical Complex have potential to degrade phenol. Also, successful biodegradation efficiency of the isolated strain may be accomplished during the process of optimization.

 
Keyword(s): BACTERIA, BIOREMEDIATION, PHENOL
 
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