Paper Information

Journal:   IRANIAN JOURNAL OF PUBLIC HEALTH   AUGUST 2014 , Volume 43 , Number SUPPLEMENT 2; Page(s) 47 To 47.
 
Paper: 

EVALUATION OF PYRITE REMOVAL FROM MOUTEH REFRACTORY GOLD ORE USING ACIDITHIO BACILLUS FERROOXIDANS PTCC1647 IN SEMI INDUSTRIAL SCALE

 
Author(s):  MOBINI MASOUD*, ASGHAR HEYDARI MARYAM, SALEHI MOHAMMAD
 
* YOUNG RESEARCHERS AND ELITE CLUB, TONEKABON BRANCH, ISLAMIC AZAD UNIVERSITY, TONEKANBON, IRAN
 
Abstract: 

Background: The aim of this work is to evaluate the pyrite remove from mouteh gold ore by A. ferrooxidans PTCC1647 in column.
Methods: In the first step, Acidithio Bacillus Ferrooxidans PTCC1647 purchased from (Iranian Research Organization for Science and Technology (IROST) was cultivated in the routinely 9k medium. In the second step, the cells suspensions (2x108 cells/ml) were used as inoculum at the 10 %v/v for column bioleaching. then column (75 cm/7.5 cm) was charged with 4Kg of sulfide gold ore from Mouteh gold mine isfahan province (Iran) with particle size 45 mesh (3.36 mm) containing FeS2 (pyrite) 78 %. 9K medium with initial pH 1.80 Containing pure cultures of A. Ferrooxidans PTCC1647 was pumped from the feed container to the top of the column. Air was pumped from Top of the column into holes embedded within the column. Experiments were carried out at 30oC for 60 days with pumping speed 1.8 l/h. Moreover, sterile column was prepared as the uninoculated control system. Redox potential, pH, ferrous iron concentaration (by spectrophotometer using 1, 10 orthophenanthroline ferrous complex as an indicator) and Sulfate concentration (indirectly determined by atomic absorption spectroscopy analysis of Ba after precipitation of BaSO4) were measured daily. The chemical composition and particle size distribution of ore was determined after bioleaching experiments.
Results: A. Ferrooxidans reduced the amount of ferrous ion form from 18.9 to 0.45 g/L. A decrease in pH (due to the oxidation of sulfide and sulfuric acid) and the concentration of Fe2 + (ferric iron due to oxidation) was initiated on the 14th day, and then continued until day 60. Ferrous iron to ferric iron completely was oxidized in 60 days. In chemical control flasks, only a negligible amount of ferrous iron was oxidized due to air-oxidation under the same experimental condition. XRD analysis showed that mineral pyrite was removed from ore after 60 days.
Conclusion: As result of long-term processes, a simultaneous use of these bacteria will give a better result. Since biological leaching processes in a semi-industrial experiment is considered as an introduction for industrial processes, the results of this research can be used in bio-oxidation of Mouteh refractory gold ore by industrial methods.

 
Keyword(s): BIOLACHING, REFRACTORY GOLD, COLUMN
 
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