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Paper Information

Journal:   IRANIAN JOURNAL OF PUBLIC HEALTH   AUGUST 2014 , Volume 43 , Number SUPPLEMENT 2; Page(s) 46 To 46.
 
Paper: 

SCREENING OF ACTINOMYCETES FROM LIPAR AREA OF GULF OF OMAN TO RESEARCH THE ANTIMICROBIAL COMPOUNDS

 
 
Author(s):  SHAMS MAHSA*, SHAHNAVAZ BAHAR, GHAZVINI KIARASH, VALINASAB TORAJ
 
* DEPARTMENT OF BIOLOGY, FACULTY OF SCIENCE, FERDOWSI UNIVERSITY, MASHHAD, IRAN
 
Abstract: 

Background: Actinomycetes are one of the most important sources for producing antimicrobial compounds and the most important prokaryotic microorganisms in this regard. Diversity of antimicrobial compounds isolated from terrestrial Actinomycetes has been declining and considering the increasing resistance of bacteria to antibiotics compounds, searching for new sources is necessary. Marine environment due to its unique characteristics is considered as a good option to search for bacteria with the capability of producing antimicrobial compounds.
Methods: To evaluate the antimicrobial compounds from Actinomycetes, water samples were obtained from the Gulf of Oman (Particularly, from Lipar area). To maximize the isolation of Actinomycetes, the process was done using starch-casein agar, Starch Nitrate Agar and Glycerol Glycine Agar with two different salt concentrations. To investigate the Antimicrobial production activity, the isolated Actinomycetes were assessed against reference and pathogenic gram positive strains like Staphylococcus epidermidis, S.intermedius and Methicillin resistant S.aureus and gram negative strains like Pseudomonas, Listeria, Klebsiella, Salmonella, Acinetobacter and E. coli O157 by cross streak method.
Results: 35 isolated cases belong to the Actinobacteria , based on morphological characteristics, pigment production and aerial mycelium and that %94 of these bacteria have the ability to produce antimicrobial compounds. Most of the isolated bacteria have antimicrobial compounds against reference S.aureus among gram positive bacteria and Listeria among gram negative bacteria. Inhibition zone is measured between 2 to 25 mm in diameters for garm positive bacteria and 1-20 mm in diameters for gram-negative bacteria.
Conclusion: Native Iranian Actinobacteria could be considered as suitable options to screen the new antimicrobial compounds. Molecular research and antimicrobial compounds extraction against the aforementioned pathogenic strains, are also being conducted.

 
Keyword(s): ACTINOMYCETES , ANTIMICROBIAL COMPOUNDS, LIPAR AREA
 
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