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Paper Information

Journal:   IRANIAN JOURNAL OF PUBLIC HEALTH   AUGUST 2014 , Volume 43 , Number SUPPLEMENT 2; Page(s) 46 To 46.
 
Paper: 

PRODUCTION OF PECTINASE BY ASPERGILLUS NIGER USING SOLID-STATE SUBSTRATES

 
 
Author(s):  ASL SOLEIMANI ELHAM, GHEIBI SIAMAK
 
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Abstract: 

Background: The purpose of this research was to optimize the production of pectinase by solid substrate fermentation using lignocellulosic wastes (wheat straw, rice straw) and pectic substance such as citrus pulp (lemon, orange).
Methods: Aspergillus niger PTCC 5010 was obtained from Iranian Research Organization for Science and Technology (IROST). Taguchi design was employed for screening the most significant factors affecting the pectinase production by strain under study. The effect of addition of various solidstate substrates such as wheat straw, rice straw, orange pulp and lemon pulp, pulp ratio, carbon/ nitrogen (C/N) ratio, and pH was studied for optimal pectinase production. Pectinase assay was done spectrophotometrically using galacturonic acid (GaiA) as the substrate.
Results: In present study mixed of wheat and rice straws was utilized in combination with orange and lemon pulp with various percentages to product the enzyme with Aspergillus niger. The results showed that the highest pectinase activity was obtained using lemon pulp and orange pulp with maximum activity of 7819.15 U/mg. In addition the maximum pectinase production in the pulp of oranges and lemons was obtained at C/N ratio of 2 and 1%, respectively. Also the highest enzyme activity in orange pulp and lemon pulp was found at PH=6.
Conclusion: The great amount of pulps daily produced during citrus juice processing makes their elimination difficult through a single system. Unprocessed pulps could be partially utilized as components of animal feed mixtures or source of human dietary fibers, whose world demand is increasing. Pulps could be alternatively utilized as carbon sources to grow microorganisms. Result indicated that increase for pulp as a solid substrate for the enzyme production was increased. This study showed that citrus pulp due to its high pectin is a potential source for increasing the production of pectinase.

 
Keyword(s): PECTINASE, ASPERGILLUS NIGER, SOLID-STATE FERMENTATION, CITRUS PULP
 
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