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Paper Information

Journal:   IRANIAN JOURNAL OF PUBLIC HEALTH   AUGUST 2014 , Volume 43 , Number SUPPLEMENT 2; Page(s) 45 To 45.
 
Paper: 

MOLECULAR DETECTION OF MICROCYSTINE PRODUCING IN WATER OF PERSIAN GULF, HORMOZGAN PROVINCE WITH PCR METHOD

 
 
Author(s):  YAZDANPANAH ABDOLMALAKI PEGAH
 
* 
 
Abstract: 

Background: This study aimed to diagnose the molecule which produces Microcystine gene in Cyanobacterias of Persian Gulf in Hormozgan using PCR method.
Methods: A paire of primers, PC-R and PC-F, were used to diagnose Cyanobacteria’s and other paire of primers, Mcya- Cd1R and Mcya-Cd1F were used for diagnosing the gene which produced Toxin Microcystine A mcyA. Then, PCR test was gotten the optimum and allergy and specialty test were also done. The product of increased gene was transformed by plasmid Ptz257 in E. coli bacteria (JM1.7). 20 samples of water were prepared from various stations in Persian Gulf and consequently, by using transformed DNA methodology, DNG was extracted and the presence of Cyanobacteria and background : This study aimed to diagnose the molecule which produces Microcystine gene in Cyanobacterias of Persian Gulf in Hormozgan using PCR method.
Methods: A paire of primers, PC-R and PC-F, were used to diagnose Cyanobacterias and other paire of primers, Mcya- Cd1R and Mcya-Cd1F were used for diagnosing the gene which produced Toxin Microcystine A mcyA. Then, PCR test was gotten the optimum and allergy and specialty test were also done. The product of increased gene was transformed by plasmid Ptz257 in E. coli bacteria (JM1.7). 20 samples of water were prepared from various stations in Persian Gulf and consequently, by using transformed DNA methodology, DNG was extracted and the presence of Cyanobacteria and Cyanobacterias generating Toxin was investigated.
Results: Optimums PCR for primers with the length of 650bp and Toxin Microcystine with the length of 297bp were increased and then they were observed by Electrophorese gell. Using specialty test by various primers with DNA, related to eight Microorganisms, proved 100% of their specialty. The presence of Cyanobacteria in all 20 stations was admitted but the presence of Cyanobacterias generating Toxin was admitted in three stations.
Conclusion: In diagnosing Cyanobacteria and the strains of Toxin generator, PCR methodology was faster and more accurate than traditional methods. This method can also be used to find the certain presence of the Cyanobacterias generating Toxin.

 
Keyword(s): MICROCYSTINE, PCR, CYANOBACTERIA, PERSIAN GULF
 
 
References: 
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Citations: 
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APA: Copy

YAZDANPANAH ABDOLMALAKI, P. (2014). MOLECULAR DETECTION OF MICROCYSTINE PRODUCING IN WATER OF PERSIAN GULF, HORMOZGAN PROVINCE WITH PCR METHOD. IRANIAN JOURNAL OF PUBLIC HEALTH, 43(SUPPLEMENT 2), 45-45. https://www.sid.ir/en/journal/ViewPaper.aspx?id=410154



Vancouver: Copy

YAZDANPANAH ABDOLMALAKI PEGAH. MOLECULAR DETECTION OF MICROCYSTINE PRODUCING IN WATER OF PERSIAN GULF, HORMOZGAN PROVINCE WITH PCR METHOD. IRANIAN JOURNAL OF PUBLIC HEALTH. 2014 [cited 2021October16];43(SUPPLEMENT 2):45-45. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=410154



IEEE: Copy

YAZDANPANAH ABDOLMALAKI, P., 2014. MOLECULAR DETECTION OF MICROCYSTINE PRODUCING IN WATER OF PERSIAN GULF, HORMOZGAN PROVINCE WITH PCR METHOD. IRANIAN JOURNAL OF PUBLIC HEALTH, [online] 43(SUPPLEMENT 2), pp.45-45. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=410154.



 
 
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