Paper Information

Journal:   IRANIAN JOURNAL OF PUBLIC HEALTH   AUGUST 2014 , Volume 43 , Number SUPPLEMENT 2; Page(s) 43 To 43.
 
Paper: 

OPTIMIZATION OF BENZENE BIODEGRADATION USING STREPTOMYCES ISOLATED FROM TABRIZ REFINERY

 
 
Author(s):  SHIRZAD NAJIBEH*, FARZI ALI, DEHNAD ALI REZA, HAJIZADEH NADER
 
* YOUNG RESEARCHER AND ELITE CLUB, AHAR BRANCH, ISLAMIC AZAD UNIVERSITY, AHAR, IRAN
 
Abstract: 

Background: Streptomyces bacteria isolated from soil of Tabriz Refinery was used for biodegradation of benzene. Then kinetic modeling of biodegradation and optimization of environmental conditions and pH were performed.
Methods: After isolation and screening, effective species for degradation of benzene were detected. Then bacteria were cultured in Muller Hinton Agar. Mineral medium was used for experiments. In addition to benzene, bacteria were added to one of the samples and by comparison of mineral medium containing only benzene (control environment); the changes resulting from the influence of bacteria were investigated. The ability of bacteria to decompose benzene was determined by maximum wavelength using spectrophotometer. Further experiments were carried out using gas chromatography. After extracting experimental data, the reaction kinetic model for biodegradation was proposed and optimization of the concentration, temperature and pH was performed.
Results: Bacteria can play an important role in bioremediation and cleaning hydrocarbon contaminated soils due to their ability for biodegradation of benzene. This bacterium is capable of decomposing benzene up to 70% while this method does not produce harmful excipients. According to the experimental data and proposed kinetic model, reaction obeys a first-order-reaction kinetic. Optimal conditions include: PH 5; temperature 25 C.
Conclusion: The identified bacterium has an acceptable performance in biodegradation of benzene and can degrade it up to 70%. On the other hand, Streptomyces is indigenous and resistant to environmental and can be used in these fields with variable climate changes. The effect of Streptomyces on biodegradation of other aromatic hydrocarbons can be studied for further researches.

 
Keyword(s): BENZENE, STREPTOMYCES, BIODEGRADATION, REACTION KINETIC MODEL, OPTIMIZATION
 
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