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Journal:   IRANIAN JOURNAL OF PUBLIC HEALTH   AUGUST 2014 , Volume 43 , Number SUPPLEMENT 2; Page(s) 43 To 43.
 
Paper: 

Preparation And In Vitro Evaluation Of Antitumor Activity Of Tgf?L3-Seb As A Ligand-Targeted Superantigen

 
 
Author(s):  Yousefi Forough*, Musavi Seyyed Fazlollah, Siadat Seyed Davar, Imani Fooladi Abbas Ali, Amani Jafar, Aslani Mohammad Mehdi, Azizi Saraji Alireza
 
* DEPARTMENT OF MEDICAL BACTERIOLOGY, PASTEUR INSTITUTE OF IRAN, TEHRAN, IRAN
 
Abstract: 
Background: In this study, we explored the antitumor potency of tumor-targeted super antigens (TTS) strategy; we designed and produced a fusion protein as a new antitumor candidate by fusing the third loop (L3) of transforming growth factor ? (TGFaL3) genetically to staphylococcal enterotoxin type B and evaluated in vitro antitumor activities against murine breast cancer cell line.Methods: We designed and prepared TGFaL3-SEB chimeric protein and evaluated superantigenic activity, binding property to cancer cell over expressing epidermal growth factor receptor (EGFR) and in vitro antitumor activities.Results: Cloning of tgfal3-seb was confirmed by colony- PCR, enzymatic digestion and sequencing. The recombinant TGFaL3-SEB fusion protein with molecular weights of 31 kDa was expressed and confirmed by anti-his western-blot analysis. TGFaL3-SEB fusion protein attached to A431 cell line with proper affinity and induced dose-dependent cytotoxicity against cancer cells expressing EGFR in vitro.Conclusion: TGFaL3-SEB fusion protein was successfully designed, expressed and purified. This chimeric fusion protein exhibits potent in vitro antitumor activity. So, these results indicate that TGFaL3-SEB might be a promising anticancer candidate for cancer immunotherapy and further efforts are needed to explore this potential therapeutic strategy.
 
Keyword(s): BREAST CANCER, STAPHYLOCOCCAL ENTEROTOXIN TYPE B (SEB)
 
 
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APA: Copy

YOUSEFI, F., & MUSAVI, S., & SIADAT, S., & IMANI FOOLADI, A., & AMANI, J., & ASLANI, M., & AZIZI SARAJI, A. (2014). PREPARATION AND IN VITRO EVALUATION OF ANTITUMOR ACTIVITY OF TGF?L3-SEB AS A LIGAND-TARGETED SUPERANTIGEN. IRANIAN JOURNAL OF PUBLIC HEALTH, 43(SUPPLEMENT 2), 43-43. https://www.sid.ir/en/journal/ViewPaper.aspx?id=410107



Vancouver: Copy

YOUSEFI FOROUGH, MUSAVI SEYYED FAZLOLLAH, SIADAT SEYED DAVAR, IMANI FOOLADI ABBAS ALI, AMANI JAFAR, ASLANI MOHAMMAD MEHDI, AZIZI SARAJI ALIREZA. PREPARATION AND IN VITRO EVALUATION OF ANTITUMOR ACTIVITY OF TGF?L3-SEB AS A LIGAND-TARGETED SUPERANTIGEN. IRANIAN JOURNAL OF PUBLIC HEALTH. 2014 [cited 2022August08];43(SUPPLEMENT 2):43-43. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=410107



IEEE: Copy

YOUSEFI, F., MUSAVI, S., SIADAT, S., IMANI FOOLADI, A., AMANI, J., ASLANI, M., AZIZI SARAJI, A., 2014. PREPARATION AND IN VITRO EVALUATION OF ANTITUMOR ACTIVITY OF TGF?L3-SEB AS A LIGAND-TARGETED SUPERANTIGEN. IRANIAN JOURNAL OF PUBLIC HEALTH, [online] 43(SUPPLEMENT 2), pp.43-43. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=410107.



 
 
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