Paper Information

Journal:   IRANIAN JOURNAL OF PUBLIC HEALTH   AUGUST 2014 , Volume 43 , Number SUPPLEMENT 2; Page(s) 41 To 41.
 
Paper: 

INVESTIGATION OF TIGECYCLINE RESISTANCE AMONG METALLO-BETALACTAMASE PRODUCING ACINETOBACTER BAUMANNII ISOLATES IN AN EDUCATIONAL HOSPITAL OF ESFAHAN

 
 
Author(s):  FARHANG ARMIN*, ZANDI HENGAMEH, ESLAMI GILDA, SHOKRI DARIUSH
 
* DEPARTMENT OF MICROBIOLOGY, FACULTY OF MEDICINE, SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES, YAZD, IRAN
 
Abstract: 

Background: This study was designed to investigate the tigecycline resistance among metallo-beta-lactamase producing Acinetobacter baumannii isolates in an educational hospital of Esfahan.
Methods: In this cross-sectional study, 107 A. baumannii recovered from trachea, bronchial, wound urine; were identified by standard biochemical tests; and conformed as Acinetobacter baumannii species using blaOXA-51- like gene and PCR method. Antimicrobial susceptibility to 15 antibiotics was performed using Kirby-Bauer disc diffusion method; also, strains of A. baumannii non-susceptible to imipenem were screened for MBL production. Determination of minimum inhibitory concentration to colistin sulfate was carried out using E-test method. Combination disk was used for detection of MBL production. A solution was prepared by dissolving 186.1 g of disodium EDTA.2H2O in 1, 000 mL of distilled water and adjusting it to PH 8.0. Two 10
mg imipenem disks were placed on the plate, and 10 ml of a 0.5 M EDTA solution was added to one of them. Increasing of inhibition zone with imipenem-EDTA ³5 mm was determined as MBL producing isolate. According to EUCAST interpretative criteria, diameter of inhibition zone ³8 mm sensitive and 15>mm resistant were considered.
Results: Totally, 100 isolates (93.45%) were MBL producers. Most of the isolates were obtained from the intensive care unit (68%) and the isolates were obtained from different clinical specimen; which were obtained from trachea (40%), bronchial (14%), wound (12%), urine (11%) and others (23%). Resistance rates were 99%, 96%, 85%, 79% and 56% against ceftazidime, imipenem, gentamicin, trimethoprim/ sulfamethooxazole and amikacin respectively. The results revealed that 70% of our isolates were strongly sensitive to tigecycline and 26% were intermediately sensitive. No isolate was resistant to colistin sulfate.
Conclusion: The antibiotic resistance to most of the antibiotics is very high and resistance against tigecycline is increasing which can be caused by over expression of efflux pumps. In addition, colistin sulfate was the most effective antibiotic to be used in A. baumannii infections.

 
Keyword(s): ACINETOBACTER BAUMANNII, METALLO-BETA-LACTAMASES, TIGECYCLINE
 
References: 
  • ندارد
 
  Yearly Visit 54
 
Latest on Blog
Enter SID Blog