Paper Information

Journal:   IRANIAN JOURNAL OF PUBLIC HEALTH   AUGUST 2014 , Volume 43 , Number SUPPLEMENT 2; Page(s) 41 To 41.
 
Paper: 

BIOFILM FORMATION AND ANTIBIOTIC RESISTANCE OF ACINETOBACTER SPP. ISOLATED FROM SKIN AND WOUND INFECTIONS

 
 
Author(s):  AZEMAT ESLAMTALAB ELNAZ*
 
* DEPARTMENT OF MICROBIOLOGY, ISLAMIC AZAD UNIVERSITY, KERMAN BRANCH, KERMAN, IRAN
 
Abstract: 

Background: Some bacteria cause severe infections in compromised patients and they survive on abiotic surfaces in hospital environments and colonize on different medical devices. On this basis, samples of skin and wound infections were collected from three hospitals in Kerman, Iran.
Methods: All isolates were characterized with conventional biochemical methods. Antibiotic susceptibility of all isolates was carried out with nineteen antibiotics from different groups by disc diffusion technique. Minimum inhibitory concentration (MIC) of seven antibiotics was determined against all isolates by agar dilution method (1-1024)
mg/ml. The cell surface hydrophobicity (CSH) of all isolates was determined and four isolates with the highest CSH were evaluated for biofilm formation on different surfaces such as glass, polycarbonate, polypropylene and venous catheters.
Results: Twenty three Acinetobacter spp. were identified. According to Antibiotic susceptibility tests, all Acinetobacter strains were resistant to Cephalosporins and Quinolons (95.6%), Aminoglycosides (91.3%), Sulfanamids (95.6%), Tetracyclin (47.8%), Carbapenems (100%) and Colistin (8.6%). MIC of seven antibiotics except Colistin against all isolates was more than 128 ?g/ml. Biofilm formation of the four selected isolates on glass and polypropylene tubes indicated denser aggregates on polypropylene than glass surfaces. The number of bacteria that adhered to venous catheter surface was reduced after treatment of culture with Colistin.
Conclusion: One of the isolates with highest biofilm formation was identified by 16S rRNA technique as Acinetobacter baumannii Iliya and registered as a new strain, which is a nosocomial agent, and its high colonization activity on medical devices was precisely proved in this research.

 
Keyword(s): WOUND, ANTIBIOTIC SENSITIVITY, HYDROPHOBICITY, BIOFILM FORMATION
 
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