Paper Information

Journal:   IRANIAN JOURNAL OF PUBLIC HEALTH   AUGUST 2014 , Volume 43 , Number SUPPLEMENT 2; Page(s) 40 To 40.
 
Paper: 

EVALUATION OF THE PATTERN OF ANTIBIOTIC RESISTANCE AND INCIDENCE OF BROADSPECTRUME BETALACTAMASES TYPE VEB AND INTEGRON CLASS1 AT ACINETOBACTER ISOLATIONS SEPARATED FROM CLINICAL SPECIMEN AT EDUCATIONA

 
Author(s):  KHOLDI SOUDEH*
 
* DEPARTMENT OF MICROBIOLOGY, STUDENT RESEARCH COMMITTEE, FACULTY OF MEDICINE, MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, SARI, IRAN
 
Abstract: 

Background: Acinetobacter has emerged as a significant opportunistic pathogen responsible for nosocomial infections. Treatment of infections due to this organism is becoming a serious clinical concern and these bacteria are frequently resistant to multiple classes of antibiotics such as Family of beta-lactam drugs. The b-lactamase enzymes represent the main mechanism of bacterial resistance to b-lactam antibiotics. This study was conducted to determine the prevalence of VEB and INT 1 in Acinetobacter isolates from sari hospital.
Methods: The study included 100 Acinetobacter isolates that were isolated from various clinical specimens. Susceptibility of isolates to the antibiotics was determined by standard disk diffusion method. ESBL production was determined by combination disk method. Using disks containing ceftazidim and cefotaxim alone and incombination with Clavulanic acid and VEB and Integron class1 producing genes was detected by PCR test.
Results: Among all Acinetobacter isolates, the highest resistance was observed for cefotaxime (100%), ceftazidim (100%), ceftraiaxone (96%), whereas the highest susceptibility was observed forcolistin (65%) Gentamycin (37%), tobramaycin (27%). Combined Disc Test showed that 24% of isolated were ESBL positive and among them 16.6% and 75% were positive for blaVEB and INT1 genes.
Conclusion: According to the results, most of the isolates of acintobacter are drug resistant. The number of isolates of
b lactamas producer is 24% of the total samples. Thus, other mechanisms such as secretory pump and purines can have a role in drug resistance.

 
Keyword(s): ACINETOBACTER, ESBL, ANTIMICROBIAL SESISTANT, BLAVEB, INTEGRON CLASS 1
 
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