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Paper Information

Journal:   IRANIAN JOURNAL OF PUBLIC HEALTH   AUGUST 2014 , Volume 43 , Number SUPPLEMENT 2; Page(s) 39 To 39.
 
Paper: 

MOLECULAR IDENTIFICATION OF ESBL GENES BLA-SHV, BLA-VEB, AND BLA-PER, IN ACINETOBACTER BAUMANNII ISOLATED FROM PATIENTS ADMITTED IN A UNIVERSITY HOSPITAL IN ISFAHAN

 
 
Author(s):  FAZELI HOSSEIN, TARAGHIAN AZADEH*, POURSINA FARKHONDEH, KAMALI RAZIE
 
* DEPARTMENT OF MICROBIOLOGY, SCHOOL OF MEDICINE, ISFAHAN UNIVERSITY OF MEDICAL SCIENCES, ISFAHAN
 
Abstract: 

Background: Acinetobacter baumannii has become one of the most important pathogens responsible for healthcare ? associated infections and particularly affects patients admitted to intensive care units (ICUs). Prevalence of class extended-spectrum -lactamases (ESBLs) has been investigated in Acinetobacter baumannii. The aims of this study were to determine the prevalence of ESBL genes bla SHV, bla VEB, and bla PER in clinical isolates of Acinetobacter baumannii in a university hospital in Isfahan.
Methods: Within 8 mounths, 121 clinical isolates of A. baumannii were collected from Alzahra Hospital in Isfahan. To confirm the species of baumannii a PCR test for identification of blaoxa-51 genes was conducted. Antimicrobial susceptibility was done by testing resistance to cefotaxime, ceftriaxone, ampicillin-sulbactam, cefepime, meropenem, tobramycin, amikacin, tetracycline, ciprofloxacin, trimethoprim sulfamethoxazole, Aztreonam by using the Kirby Bauer disk diffusion method. Finally all of isolates were evaluated by PCR for detection of bla-SHV, bla-PER and bla-VEB genes.
Results: Among 121 isolates in this study 44% were female and 56% were male. Samples were cultured from the Trachea (35%), urine (17%), and blood (10%). Most of the isolates (50%) were from ICU. Resistance rate to antibiotics was high: cefotaxime (100%), ceftriaxone (100%), ampicillinsulbactam (33.9%), cefepime (99.2%), meropenem (100%), tobramycin (86.8%), amikacin (87.6%), tetracycline (92.6%), ciprofloxacin (100%), trimethoprim-sulfamethoxazole (99.2%), Aztreonam (100%). 62.8% isolates were XDR and 100% were MDR. Among all samples bla -SHV gene was detected in 16%, bla-VEB in 26.6% and bla-PER in 36.8% of isolates.
Conclusion: The result of this study shows the growing number of nosocomial infection associated with XDR A. baumannii complex leading to difficulties in antibiotic therapy. In this study Ampicillin-sulbactam with resistant rate of 33.9% was the most effective antibiotic. Also this study confirms the large dissemination of the gene bla-PER among A. baumannii in Alzahra Hospital and the results of antibiotic susceptibility revealed high rates of resistance to different groups of antibiotics.

 
Keyword(s): ACINETOBACTER BAUMANNII, DRUG RESISTANCE, ESBL, BLA -SHV, BLA-VEB, AND BLA-PER
 
References: 
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