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Paper Information

Journal:   IRANIAN JOURNAL OF PUBLIC HEALTH   AUGUST 2014 , Volume 43 , Number SUPPLEMENT 2; Page(s) 37 To 37.
 
Paper: 

ANTIMICROBIAL ACTIVITY OF ENDOPHYTIC BACTERIAL POPULATIONS ORIGINATED FROM MEDICAL PLANTS IN IRAN

 
 
Author(s):  AMIN MANSOUR, BIERANVAND MARYAM*, HASHEMI SHAHRAKI ABDOLRAZAAGH
 
* DEPARTMENT OF MICROBIOLOGY, SCHOOL OF MEDICINE, HEALTH RESEARCH INSTITUTE, INFECTIOUS AND TROPICAL DISEASES RESEARCH CENTER, AHVAZ JUNDISHAPUR UNIVERSITY OF MEDICAL SCIENCES, AHVAZ, IRAN
 
Abstract: 

Background: The aim of this study was the isolation, molecular identification and antimicrobial activity of endophytic bacteria isolated from medicinal plants in some areas of Iran.
Methods: From April to Jun 2013 and April to May 2014, 23 fresh plant samples were collected. The samples were washed in running tap water to remove the surface soils. After drying, the samples were subjected to five step surface sterilization procedure. The surface- sterilized tissues were aseptically crumbled into small fragments with a scalpel then cultured directly on the R3A, ISP2 and Blood agar plates. The plates were incubated at 28oC for 2 to 4 days. Each isolate was cultured in TSB medium and incubated at 28oC for 14 to 16 days then the antimicrobial compound was extracted through 3 methods. The extractions were tested against target bacteria. Method 1: just using a part of culture medium. Method 2: using hot. Method 3: using an ultrasonic device.
Results: From 23 medical plants, total of 23 bacteria were isolated. The isolates were identified by 16S rRNA gene sequencing as species level. The most abundant genera among the isolates were Bacillus. All isolates were used for extraction of their antimicrobial compound. The extractions obtained by method 2 and method 3 were tested against the target bacteria. Species belong to Actinomycets family made up the largest fraction of the isolates. Using method 1, just 2 of 23 isolates had an activity against the pathogenic microorganism. With method 2, sixty of 23 isolates (69%) exhibited activity against the tested pathogenic microorganisms. 43 isolates were active against Bacillus cereus and 26% were active against Staphylococcus aureus, 34% were active against Bacillus subtilis and 29.9% strongly active against Klebsiella pneumonia, 17% were active against Citrobacter freundii, 17% were active against Proteus mirabilis, 47/6% were active against Shigella and 17% were active against Escherichia coli. Thirteen out of 23 isolates (46%) exhibited activity against the tested pathogenic microorganisms with method 3. 16.6% were active against Bacillus subtilis and 34% were active against Klebsiella pneumonia. 34% were active against Citrobacter freundii. 34% were active against Shigella.
Conclusion: 16S rRNA technique was useful to identify isolates as species level.

 
Keyword(s): ENDOPHYTIC BACTERIAL, PLANT, IRAN
 
 
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APA: Copy

AMIN, M., & BIERANVAND, M., & HASHEMI SHAHRAKI, A. (2014). ANTIMICROBIAL ACTIVITY OF ENDOPHYTIC BACTERIAL POPULATIONS ORIGINATED FROM MEDICAL PLANTS IN IRAN. IRANIAN JOURNAL OF PUBLIC HEALTH, 43(SUPPLEMENT 2), 37-37. https://www.sid.ir/en/journal/ViewPaper.aspx?id=410039



Vancouver: Copy

AMIN MANSOUR, BIERANVAND MARYAM, HASHEMI SHAHRAKI ABDOLRAZAAGH. ANTIMICROBIAL ACTIVITY OF ENDOPHYTIC BACTERIAL POPULATIONS ORIGINATED FROM MEDICAL PLANTS IN IRAN. IRANIAN JOURNAL OF PUBLIC HEALTH. 2014 [cited 2021November27];43(SUPPLEMENT 2):37-37. Available from: https://www.sid.ir/en/journal/ViewPaper.aspx?id=410039



IEEE: Copy

AMIN, M., BIERANVAND, M., HASHEMI SHAHRAKI, A., 2014. ANTIMICROBIAL ACTIVITY OF ENDOPHYTIC BACTERIAL POPULATIONS ORIGINATED FROM MEDICAL PLANTS IN IRAN. IRANIAN JOURNAL OF PUBLIC HEALTH, [online] 43(SUPPLEMENT 2), pp.37-37. Available: https://www.sid.ir/en/journal/ViewPaper.aspx?id=410039.



 
 
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