Paper Information

Journal:   IRANIAN JOURNAL OF PUBLIC HEALTH   AUGUST 2014 , Volume 43 , Number SUPPLEMENT 2; Page(s) 36 To 36.
 
Paper: 

STUDY OF EXPRESSION OF THE GENE ALPHA-6 IN MULTIDRUG-RESISTANT ACINETOBACTER BAUMANNII AGAINST THYME ESSENCE WITH REAL TIME PCR

 
 
Author(s):  SAGHI HOSSEIN*, ESMAEILI DAVOUD, BAHADOR ABBAS, KHALEDI AZAD, AMIRI DASTJERDI FERDOES
 
* MOLECULAR BIOLOGY RESEARCH CENTER, AND MICROBIOLOGY DEPARTMENT, BAQIYATALLAH UNIVERSITY MEDICAL OF SCIENCES, IRAN
 
Abstract: 

Background: The present study was aimed to investigate the inhibitory effects of Thyme essence on the expression of antibiotic resistance genes aphA-6 and Housekeeping DNA gyrase -A against Multidrug-resistant strains of Acinetobacter baumannii with Real time PCR technique.
Methods: Five Multidrug-resistant strains of Acinetobacter baumannii among seventy-five drug-resistant strains of Acinetobacter baumannii selected from hospitals in Tehran. AphA- 6 and Housekeeping DNA Gyrase-A genes with PCR method were approved for studies. Acinetobacter baumannii ATCC
 #19606 was used in this study as a model reference strain. MIC values were determined by broth micro dilution assay recommended by the NCCLS. Thyme essence to the MIC was added to 1 ml Muller Hinton broth and after mixing, 1 ml of bacterial suspension (5×10 5 CFU/ml) was added to the medium, incubated at 37oC for 24 hours. After incubating bacteria, bacterial mRNA was extracted and transformed into cDNA and level of expression of aphA-6 and Housekeeping DNA gyrase-A genes in comparison to non-exposed Thyme essence was examined by Real time PCR. Results: The major components of Thyme essence were thymol (28.8%) and carvacrol (23.46%). Thyme essence with MIC (0.45 l/ml) and an inhibitory effect on multidrug resistant A.bummanii was found. The average zone of inhibition by Thyme essence on multidrug-resistant A.bummanii growth was 18.6 mm. Antibiotic susceptibility test results among seventy five A.bummanii strains are Oxacillin (100%), amikacin (75%), kanamycin (68)%, gentamicin (60%), imipenem (60%) and (89%) were resistant to neomycin. Melting curve analysis showed the species- specific melting temperature patterns on 53o? Differentiating A. bummanii. Thyme essence with MIC (0.45?1 / ml) has the effect of reducing the expression of antibiotic resistance genes aphA-6 with Real time PCR method and no inhibitory effect on Housekeeping DNA Gyrase-A gene.
Conclusion: Thyme essence has strong inhibitory effects against Acinetobacter baumani. Therefore, due to the increasing resistance of pathogenic bacteria, Thyme essence can be used as a natural alternative for common antiseptic. Additional clinical researches are necessary to completely confirm the above results for practical purposes.

 
Keyword(s): ACINETOBACTER BAUMANNII, THYME, APHA-6GENE
 
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