Paper Information

Journal:   IRANIAN JOURNAL OF PUBLIC HEALTH   AUGUST 2014 , Volume 43 , Number SUPPLEMENT 2; Page(s) 35 To 35.
 
Paper: 

IDENTIFICATION AND DRUG SENSITIVITY OF ACINETOBACTER BAUMANNII CLINICAL ISOLATES FROM AHVAZ AND TEHRAN

 
 
Author(s):  SADEGHI PARISA*, KHOSRAVI AZAR DOKHT
 
* DEPT. OF MICROBIOLOGY, SCHOOL OF MEDICINE, SHAHREKORD UNIVERSITY OF MEDICAL SCIENCE
 
Abstract: 

Background: Acinetobacter baumannii is an emerging opportunistic nosocomial pathogen in hospitalized patients of intensive care units. The purpose of this study was to identify the Acinetobacter spp. clinical isolates to specie level, and determine their Multi Drug Resistance (MDR) and Extensively Drug Resistance (XDR) status.
Methods: 142 clinical isolates of Acinetobacter spp from laboratories of university teaching hospitals in Ahvaz and 55 clinical isolates of Acinetobacter spp from Tehran were investigated. All of the isolates were identified as species level using rpoB gene sequencing. Susceptibility testing using disk diffusion method was performed on A. baumannii isolates to determine MDR and XDR stratus. The results were interpreted according to the Clinical Laboratory Standards Institute (CLSI) guideline.
Results: Out of total 197 Acinetobacter spp., 131 were identified as A. baumannii based on rpoB gene sequencing. Susceptibility testing revealed that 109 (55%) of A. baumannii isolates were MDR and 22 (11%) were XDR. Drug resistance rate was higher among Ahvaz isolates in comparison to isolates from Tehran.
Conclusion: Acinetobacter baumannii is an emerging opportunistic nosocomial pathogen with a high prevalence of multiple drug resistance. rpoB gene sequencing is a useful marker to assign the isolates as species level in genus of Acinetobacter.

 
Keyword(s): ACINETOBACTER BAUMANNII, RPOB SEQUENCING, MDR, XDR
 
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