Click for new scientific resources and news about Corona[COVID-19]

Paper Information

Journal:   IRANIAN JOURNAL OF PUBLIC HEALTH   AUGUST 2014 , Volume 43 , Number SUPPLEMENT 2; Page(s) 34 To 34.
 
Paper: 

CLONAL EVOLUTION OF MULTI-DRUG RESISTANT ACINETOBACTER BAUMANNII BY PULSED-FIELD GEL ELECTROPHORESIS

 
 
Author(s):  MOHAJERI PARVIZ, FARAHANI ABBAS*
 
* DEPT. OF MICROBIOLOGY, SCHOOL OF MEDICINE, AHVAZ JUNDISHAPUR UNIVERSITY OF MEDICAL SCIENCES, AHVAZ, IRAN
 
Abstract: 

Background: The aim of this study was to determine the MDR isolates and the existence of OXAs genes among MDR isolates of A. baumannii collected from Kermanshah hospitals in west of Iran.
Methods: Fourty-two MDR A. baumannii isolates were collected from patients of Kermanshah hospitals. The isolates were identified by biochemical tests and API 20NE kit. The susceptibility to different antibiotics by disk diffusion method (CLSI) was determined. PCR was performed for detection of blaOXA-23-like, blaOXA-24-like, blaOXA-51-like and blaOXA-58-like beta lactamase genes in isolates and clonal relatedness was done by PFGE (with the restriction enzyme ApaI). The patterns were then analyzed by Bionumeric software.
Results: This study showed high resistance to ciprofloxacin, piperacillin, ceftazidime and resistance to other antimicrobial agents and more spread of blaOXA-23-like gene (93%) in MDR isolates. Six clones were obtained through PFGE method named in A (10), B (9), C (5), D (4), E (11) and F (3) that clone E was an outbreak and dominant in different wards of the hospitals.
Conclusion: An isolate from the emergency ward of the hospitals had indistinguishable isolates PFGE profile and similar resistance profile to isolates from intensive care unit (ICU) proposes that the transmission from ICU to emergency ward probably occurs through patients or hospital staff contact.

 
Keyword(s): ACINETOBACTER BAUMANNII, MULTI-DRUG RESISTANT, OXA-TYPE, PULSED-FIELD GEL ELECTROPHORESIS
 
References: 
  • ندارد
 
  Yearly Visit 43
 
Latest on Blog
Enter SID Blog