Paper Information

Journal:   IRANIAN JOURNAL OF PUBLIC HEALTH   AUGUST 2014 , Volume 43 , Number SUPPLEMENT 2; Page(s) 30 To 30.
 
Paper: 

POLYMORPHISM OF COA AND AROA GENES IN THE METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS STRAINS ISOLATED FROM NASAL CARRIAGE AMONG HOSPITALIZED PATIENTS IN WEST OF IRAN

 
 
Author(s):  MOHAJERI PARVIZ*, AZIZKHANI SAMIRA, FARAHANI ABBAS
 
* DEPARTMENT OF MICROBIOLOGY, SCHOOL OF MEDICINE, KERMANSHAH UNIVERSITY OF MEDICAL SCIENCES, KERMANSHAH, IRAN
 
Abstract: 

Background: Methicillin resistant Staphylococcus aureus (MRSA) is an opportunist bacterial pathogen frequency isolated in the hospital and community environments. The main objective of this study was to determine genotyping of MRSA strains isolated from the noses of the hospitalized patients in the Kermanshah Hospital, western Iran by PCRRFLP.
Methods: 1269 isolates from patients screened more than 48 hours of admission from different wards and 330 isolates from hemodialysis word of Kermanshah Hospital as the largest hospital in western Iran. S.aureus was identified by biochemical tests common methods and Api 20 staph test (Bio Merieux) and MRSA was identified by Oxacillin strip (Mast STOX).
Results: 258 S. aureus isolates were recovered from 1387 samples. 96 of that 82 isolates hospital-acquired and 14 isolates was community-acquired. Digestion of aroA gene amplified (1, 153 bp) yielded only one distinctive RFLP pattern. All of the isolates were generated approximately 850 and 300 bp bands.
Conclusion: MRSA is an increasingly common cause of nosocomial infections. Our results are in agreement with those from others, who showed that a few specialized colons are responsible for the majority of MRSA nasal carriers. In this study, MRSA strains isolated in different wards of hospital were closely related when analyzed by coagulase gene typing.

 
Keyword(s): METHICILLIN-RESISTANT S. AUREUS, PHENOTYPIC, STAPHYLOCOCCUS AUREUS, RFLP, COA GENE, AROA GENE
 
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