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Paper Information

Journal:   IRANIAN JOURNAL OF PUBLIC HEALTH   AUGUST 2014 , Volume 43 , Number SUPPLEMENT 2; Page(s) 30 To 30.
 
Paper: 

UNIQUE BIODIVERSITY OF RADIATION RESISTANT BACTERIA ISOLATED FROM AN IRANIAN RADIOACTIVE SITE AND ANALYSIS OF THEIR PIGMENTS

 
 
Author(s):  MIRZAIE AMIR*, FALLAH MEHRABADI JALIL, AMIRMOZAFFARI NOOR, NEJADSATARI TAHER
 
* DEPARTMENT OF BIOLOGY, TEHRAN SCIENCE AND RESEARCH BRANCH, ISLAMIC AZAD UNIVERSITY, TEHRAN, IRAN
 
Abstract: 

Background: Radiation resistance has been detected dispersedly among bacteria and some microorganisms growing in natural radioactive environments. Herein, we provide data of isolation, molecular identification and pigments characterization of radiation resistant bacteria recovered from an Iranian radioactive site.
Methods: The soil samples were collected from uranium radioactive site and the physico-chemical properties of the soil samples were determined. Soil serial dilutions were cultured in Trypton-Glocose-Yeast extract agar and all distinct colonies irradiated by gamma and ultraviolet radiation at various doses using a 60Co source. The surviving bacteria were identified by morphology, biochemistry and 16S rRNA gene sequencing. In addition, bacterial pigments were analyzed by spectroscopic properties, HPLC and LC-MS systems and their antioxidant activity were assessed by DPPH activity.
Results: Among 30 pure colonies, five UV and gamma radiation resistant bacteria were isolated from a radioactive site. Phylogenetic analysis based on 16S rRNA gene sequencing reveal that the isolated strains were belonged to Micrococcus lylae, Kocuria rhizophila, Rhodococcus erythropolis and Dermacoccus nishinomiyaensis. In addition, these bacteria were coccoid in shape, Gram positive, catalase positive, mesophilic, non-motile, non-sporulating, aerobic and with yellow pigmentation. The results of HPLC and LC-MS analysis of pigments show that the bacterial pigments were belonged to novel astaxanthin. In addition, the most radio resistant bacterium was Kocuria rhizophila and it was resistant to gamma radiation (30 kGy) and UV light (400 j m-2).
Conclusion: The isolated strains are the first report on radio- resistant bacteria belonged to the different genus in Iran. We supposed that these isolates could be a candidate in industrial and bioremediation applications due to their strong antioxidant activities, pigment productions and radiation resistant properties.

 
Keyword(s): RADIATION RESISTANT, BIODIVERSITY, PHYLOGENY
 
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