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Paper Information

Journal:   HEPATITIS MONTHLY   OCTOBER 2014 , Volume 14 , Number 10; Page(s) 0 To 0.
 
Paper: 

RAPAMYCIN ENHANCES HBV PRODUCTION BY INDUCING CELLULAR AUTOPHAGY

 
 
Author(s):  HUANG WENJUAN, ZHAO FENGRONG, HUANG YING, LI XIA, ZHU SUFEI, HU QIN, CHEN WEIXIAN*
 
* DEPARTMENT OF LABORATORY MEDICINE, AFFILIATED TO THE SECOND HOSPITAL CHONGQING MEDICAL UNIVERSITY, CHONGQING, CHINA
 
Abstract: 

Background: Some reports revealed that rapamycin could reactivate HBV infection. However, the mechanism has not been clearly explained.
Objectives: In this report, we studied the mechanism by which rapamycin enhances HBV replication and expression by inducing cellular autophagy.
Materials and Methods: HepG2.2.15 cells were treated with rapamycin to induce autophagy. Autophagosomes were observed by fluorescence microscopy and transmission electron microscopy. Autophagy marker protein LC3-
II/LC3-Iwas detected by Western blotting. HBV DNA and mRNA were determined by real time PCR and Southern blotting. HBsAg was evaluated by ELISA.
Results: In HepG2.2.15 cells, HBV DNA and HBsAg increased when host cells were treated with rapamycin and the effect was reversed by autophagy inhibitor, 3-methyladenine (3-MA).
Conclusions: These results indicated a potential explanation for reactivation of HBV infection when patients with hepatitis receive rapamycin

 
Keyword(s): HBV, RAPAMYCIN, AUTOPHAGY
 
References: 
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