Paper Information

Journal:   IRANIAN JOURNAL OF PUBLIC HEALTH   AUGUST 2014 , Volume 43 , Number SUPPLEMENT 2; Page(s) 29 To 29.
 
Paper: 

INCREASING ISOLATION RATE OF MOTT IN SOUTH OF TURKEY

 
 
Author(s):  MARZI MAHDI*, KAYAR BEGUM, BOZOK TAYLAN, YAKICI GULFER, BAHADORI ALI, KARACALI AYSE, KOKSAL FATIH
 
* CUKUROVA UNIVERSITY MEDICAI FACUITY, MICROBIOIOGY DEP, CUKUROVA UNIVERSITY TROPICAI DISEASE RESEARCH AND APP. CENTER, ADANA, TURKEY
 
Abstract: 

Background: Mycobacteria other than tuberculosis (MOTT) are environmental organisms capable of causing chronic disease in humans and cause increasingly serious infections especially in immunosuppressive patients. The genus Mycobacterium currently has more than 130 species, includes highly infectios M. tuberculosis complex, M. Leprae and new important threat Mycobacterium avium complex (MAC). However all of these cases, the diagnosis of MOTT is often difficult or unconvincing and it cause false diagnosis and treatments.
Methods: The aim of the present study was to describe the diversity of MOTT by sequencing and RFLP of hsp65 gene region and compare these two methods from clinical isolates received to Tropical Disease Center Region Tuberculosis laboratory in Cukurova which has strategical location with border to iraq, syria and Iran. We analyzed 2048 Mycobacterium species from 27.511 clinical materials of 9 different cities total of 19 dispansery and 2 region hospitals patients with between January 2012 – March 2014 in Cukurova region, Turkey. MOTT included in this study were isolated from clinical specimens of 49 patients had pulmonary infection, and 5 infections cases related to other sites (lymphonod, biopsy and abscess fluid). MGIT 960 system was used for the recovery of mycobacteria from clinical specimens and MPT64 card test was used for identification. Genetic characterization to species level was determined hsp65 sequencing and RFLP.
Results: Tweenty five patients presented NTM infections, of whom (23/25) manifested pulmonary symptoms, (3/25) presented lymphadenopathy and (5/25) represented cases of healthcare-associated infections. A total of 54 species were identified and included: M. abscessus; M. bolletii; M. intracellulare M.alvei, M. porcinum, M. peregrinum were the most frequent in pulmonary. Lymphadenopathy case was caused by M. fortuitum infection. We encountered M. abscessus, and M. smegmatis in cases of healthcare associated infections. Our study is ongoing.
Conclusion: Our study showed the diversity of MOTT species in Cukurova region that associates pulmonary infections. And because of relationships and similar climate conditions Cukurova regions MOTT mapping will be represent closed country as Iran, Iraq and Syria.

 
Keyword(s): MOTT, RFLP, HSP65
 
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