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Paper Information

Journal:   IRANIAN JOURNAL OF PUBLIC HEALTH   AUGUST 2014 , Volume 43 , Number SUPPLEMENT 2; Page(s) 26 To 26.
 
Paper: 

A RECOMBINANT SUBUNIT VACCINE BASED ON THE L7/L12 AND TOMP31 PROTEINS INDUCES PROTECTION AGAINST BRUCELLA INFECTION IN BALB/C MICE

 
 
Author(s):  GOLSHANI MARYAM*, RAFATI SIMA, DASHTI AMIR, BOUZARI SAEID
 
* DEPARTMENT OF MOLECULAR BIOLOGY, PASTEUR INSTITUTE OF IRAN, TEHRAN, IRAN
 
Abstract: 

Background: Brucellosis is the most common bacterial zoonotic disease worldwide and no vaccine is available for the prevention of human brucellosis. In humans, brucellosis is mostly caused by Brucella melitensis and Brucella abortus. The Omp31 and L7/L12 are immunodominant and protective antigens conserved in human Brucella pathogens. The aim of this study was to evaluate the immunogenicity & protective efficacy of a recombinant protein vaccine encoding Brucella truncated Omp31 and L7/L12 proteins.
Methods: Bioinformatic tools were used to design the truncated Omp31 and L7/L12- TOmp31 fusion protein. The humoral/cellular immune response and protection levels against challenge with wild B. melitensis and B. abortus were evaluated in vaccine immunized mice and control groups.
Results: Vaccination of BALB/c mice with the recombinant fusion protein (rL7/L12-TOmp31) provided the significant protection level against both B. melitenisis and B. abortus. Moreover, rL7/L12-TOmp31 elicited a strong specific IgG response (higher IgG2a titers) and significant IFNgamma/ IL2 production and T-cell proliferation was also observed. The Th1 oriented response persisted for 12 weeks after final immunization.
Conclusion: rL7/L12-TOmp31 could be a new potential antigen candidate for the development of subunit vaccines against B. melitensis and B. abortus.

 
Keyword(s): BRUCELLA MELITENSIS, BRUCELLA ABORTUS, TRUNCATED OMP31, L7/L12, FUSION, RECOMBINANT PROTEIN VACCINE
 
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