Paper Information

Journal:   IRANIAN JOURNAL OF PUBLIC HEALTH   AUGUST 2014 , Volume 43 , Number SUPPLEMENT 2; Page(s) 25 To 25.
 
Paper: 

SEQUENCE VARIATION OF PERTUSSIS TOXIN S1 SUBUNIT GENE IN CLINICAL BORDETELLA PERTUSSIS STRAINS OF IRANIAN ISOLATES

 
 
Author(s):  HOSEIN POUR MARYAM*, SHAHCHERAGHI FERESHTEH, NIKBIN VAJIHEH SADAT, JANNESAR AHMADI NAZANIN
 
* DEPARTMENT OF BACTERIOLOGY AND MICROBIOLOGY RESEARCH CENTER, IRAN
 
Abstract: 

Background: According to the importance of pertussis vaccine and the lack of fundamental studies about the polymorphism of virulence genes in Iran, the aim of the present search is investigation of s1 pertussis toxin gene polymorphism on circulating strains compared to vaccine strains.
Methods: 50 nasopharyngeal Dacron swabs from patients with a clinical diagnosis of pertussis sent to pertussis reference laboratory at the Pasteur Institute of Iran in 2008-2012. The cultivation, biochemical tests, the antiserum, and PCR were used to confirm B. pertussis. Sequencing of PCR products was performed to determine ptxS1 alleles. Two strains of BP 134 and BP 18323 as vaccine strains were studied.
Results: All 50 clinical isolates of B. pertussis were studied. All strains had the dominant allele ptxS1A. There was difference between the alleles of clinical strains and vaccine strains.
Conclusion: In recent years, a significant increase has been observed in the incidence of whooping cough in the world. Allelic changes in genes of virulence factors have been caused new strains resistant to both whole-cell and a cellular vaccines. Although, whole-cell vaccine was introduced about 60 years ago and the vaccination program is administering to infants and children in Iran, we have observed a significant increase in the course of illness in recent years. Our finding about allelic shift of ptxS1 gene was similar to many European and American countries showing difference of dominant allele with vaccine strains.

 
Keyword(s): POLYMORPHISM, IRANIAN, BORDET ELLA PERTUSSIS, PERTUSSIS TOXIN
 
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