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Paper Information

Journal:   IRANIAN JOURNAL OF PUBLIC HEALTH   AUGUST 2014 , Volume 43 , Number SUPPLEMENT 2; Page(s) 24 To 24.
 
Paper: 

INTRANASAL IMMUNIZATION WITH RECOMBINANT FIMH FUSED WITH FLAGELLIN ENHANCES IMMUNITY AGAINST URINARY TRACT INFECTION

 
 
Author(s):  ASADI KARAM MOHAMMAD REZA*, HABIBI MEHRI, BOUZARI SAEID
 
* DEPARTMENT OF MOLECULAR BIOLOGY, PASTEUR INSTITUTE OF IRAN, TEHRAN, IRAN
 
Abstract: 

Background: In this study, we evaluated the humoral immunity of recombinant fusion FimH. FliC of UPEC as a vaccine candidate against UTI by intranasal route.
Methods: The constructed FimH. FliC fusion protein was purified by Ni-NTA column and then LPS of the protein was removed by Triton x114. Mice were intranasally immunized three times with 25 Tg of proteins (FimH, FliC or FimH.FliC proteins). Total IgG, IgG isotypes (IgG1, IgG2a), and IgA responses of immunized mice were measured by ELISA.
Results: All mice groups induced significant immune response against FimH and FliC as compared to control mice. Mice immunized with the fused FimH. FliC protein induced significantly higher humoral (Total IgG, IgG1, IgG2a and IgA) immune responses than with FimH alone or FimH admixed with FliC. Our results showed that based on the IgG1/ IgG2a ratios, FliC directed the anti-FimH responses preferentially towards Th2.
Conclusion: To date, there has been limited success in developing an efficacious vaccine against UTI. Among UPEC antigenic proteins, FimH was reported to be highly immunogenic and induces protective immunity against UTI in mice. Furthermore, we evaluated the adjuvant properties of FliC of UPEC strains in linking with FimH antigen. Our results indicated that the FliC as adjuvant increased FimH-specific IgG, IgG2a, IgG1 and IgA that shifted the immune responses toward a Th2 profile. Our results demonstrated the ability of the fusion protein in inducing the humoral responses.

 
Keyword(s): URINARY TRACT INFECTION, VACCINE, UROPATHOGENIC ESCHERICHIA COLI, FUSION, FIMH, FLIC
 
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