Paper Information

Journal:   IRANIAN JOURNAL OF PUBLIC HEALTH   AUGUST 2014 , Volume 43 , Number SUPPLEMENT 2; Page(s) 23 To 23.
 
Paper: 

A COMPARATIVE STUDY ON DIAGNOSTIC ASSAYS, PCR, MICROSCOPIC AGGLUTINATION TEST (MAT) AND CULTURE FOR DETECTION OF RODENT LEPTOSPIROSIS IN MAZANDARAN PROVINCE, IRAN

 
Author(s):  ESFANDIARI BEHZAD, NAHRAVANIAN HOSSEIN, POURSHAFIE MOHAMMAD REZA, KHAKI PEJVAK, MOSTAFAVI EHSAN, GOOYA MOHAMMAD MEHDI, DARVISH JAMSHID, MORADI BIDHENDI SOHEILA, GHARAKHANI MEHDI
 
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Abstract: 

Background: Leptospirosis is caused by infection with Leptospira bacteria. The infection transfers from animals (commonly rats, cattle, pigs and dogs) to humans. Transmission occurs by direct or indirect contact with serum, urine or other biological fluids of infected animals. Thus, animals (especially rodents) can act as a source of infection for humans or other animals. It has a worldwide epidemic, but most in temperate and tropical regions especially in areas with rainfall and neutral and slightly alkaline soil such as northern Iran.
Methods: Rodents were trapped in 10 different areas of Mazandaran Province during the summer 2013, and 150 rodents were captured alive. Urine and kidney samples were collected from each rodent and then, samples were used for culture in accordance for isolation of live Leptospira. The presence of Leptospira DNA was evaluated in urine and kidney samples from all mice using nested PCR method. In sera samples, antibody titers and possible types of infecting serovarieties were tested by Microscopic Agglutination Test (MAT) using a panel of 20 strains of live Leptospira as antigens. The statistical analysis of the obtained data was done using SPSS version 19. A 'Pvalue'
<0.05 was considered to be statistically significant.
Results: Live Leptospira were isolated from the kidney and urine samples of 3 mice (2%). In nested PCR method, DNA of Leptospira was found just in the one urine sample of rodent (0.7%) but it was detected from kidney samples of 16 rodents (10.7%). In addition, serological study was indicated, 21.2% of sera were positive (equal or/ higher than 1: 200) against one or more serovarieties. The dominant strain was L. serjoehardjo among 11 rodents (7.3%).
Conclusion: MAT is the better method than culture and molecular based methods (including nested-PCR) to detect the bacteria. In molecular based methods, using of kidney sample is better than urine sample and there is a higher probability for detection of bacteria. Culture method is not suitable for clinical diagnostic laboratory due to its time-consuming procedure despite the fact that living organism is isolated. This method only is used for research studies.

 
Keyword(s): LEPTOSPIRA CULTURE, LEPTOSPIROSIS, MICROSCOPIC AGGLUTINATION TEST, MAT, NESTED PCR, RODENT
 
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